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Jeffrey Chalmers

  • Associate chair and professor, Chemical & Biomolecular Eng
  • 483 CBEC
    151 W. Woodruff Ave.
    Columbus, OH 43210
  • 614-292-2727

About

Education

B.A., Westmont College, 1983 
B.S., U.C. Berkeley, 1983 
Ph.D., Cornell University, 1988

 

Top Honors

  • Cell Culture Engineering Award lecture, May 2016
  • Cell Culture Engineering Award, Engineering Conference International, 2014 (global, bi-annual award)
  • OSU College of Engineering Lumley Research Award: 2011,  2006, 2001, 1997, 1993
  • Fellow, American Associate for the Advancement of Science, 2005
  • AIChE 15C plenary lecture, 2005
  • OSU College of Engineering Harrison Award, 2003
  • Fellow, American Institute for Medical and Biological Engineering (AIMBE), 2001 
  • Distinguished Multidisciplinary Team Research Award, Ohio Agricultural Research and Development Center and The Ohio State University, 1996
  • NSF Young Investigator Award, 1992-97
  • Member, Tau Beta Pi, Phi Beta Kappa, Sigma Xi
 
RESEARCH AREAS - Chalmers Laboratory for Cell Analysis and Separation 

​Mankind is currently experiencing a revolution in our understanding of biological processes, from those occurring in "simple" bacteria to those occurring in human beings. Like other scientific revolutions of the past, rapid scientific advances lead to rapid development of engineering disciplines to assist in the understanding and application of this new knowledge. We are seeing this take place today in the development of the discipline of "bioengineering." Bioengineering is a broad term and includes the application of engineering principles to biological processes. 

My research is in the area of bioengineering. I have current interest/ projects in the broad areas of cell culture, ranging from the study of human stem and cancer cells to industrially relevant cells. I also have interest in the emerging area of functional genomics, especially as it is related to cell culture, cell separation, and cancer cells. 

One specific project involves the ability to identify and separate cells based on specific immunological markers. In collaboration with a colleague at the Cleveland Clinic Foundation, we have developed three new, patented immunomagnetic instruments. One of these instruments is an analytical device, while the other two are separation instruments. All three of these instruments have a large number of applications as indicated by the research support that includes the National Cancer Institute, the National Science Foundation, the Whitaker Foundation, and a private corporation. Some of these applications include: human stem cell separation for bone marrow transplants, isolation/separation of rare cancer cells circulating in human blood, and identification and separation of genetically engineered cells with specific phenotypes. Besides studying the engineering principles of this separation approach, exciting fundamental and applied biological questions are being addressed with respect to these isolated cells using the latest molecular techniques. 

 Another research area involves the development of better fundamental understanding and scale-up criteria for bioprocesses using animal cells. While large-scale animal cell culture has become quite common, a number of important questions/issues remain unresolved. For example, what is the optimum hydrodynamic environment for a suspended or anchorage- dependent cell? What is the mechanism of action, from a molecular point of view, of surface active agents in cell culture medium? Finally, my research group is exploring collaborations with systems research faculty to develop approaches to understand the great wealth of genetic information which is rapidly becoming available. This research will have specific applications with respect to the cancer cell separation projects as well as more general interest.

Honors

  • 20140501

    Cell Culture Engineering 2014.

  • 20110101

    Lumley Research Award.

  • 20060101

    Lumley Research Award.

  • 20050101

    15C plenary lecture.

  • 20050101

    AAAS Fellow.

  • 20030101

    Harrison Award.

  • 20010101

    Fellow.

  • 20010101

    Lumley Research Award.

  • 19980101

    Annual Research Accomplishment Award.

  • 19970101

    Lumley Research Award.

  • 19960101

    Distinguished Multidisciplinary Team Research Award.

  • 19930101

    Lumley Research Award.

  • 19920101

    National Young Investigator Award.

  • 19890101-19911201

    DuPont Young Faculty Development Award.

  • Member.

Presentations

  • "Quantification of the Hydrodynamic Forces on Suspended, Anchorage Dependent Cells." 1992, Presented at Annual AIChE Meeting,
  • "Unspecified." 1998, Presented at Scientific and Clinical Applications of Magnetic Carriers,
  • "The potential, and currently practical uses of immunomagnetic labels for detection." 1999, Presented at First NASA & NCI Workshop on Sensors for Bio-Molecular Signatures,
  • "Rapid flow cell sorting in an axisymmetric magnetic field and an annular SPLITT channel." 1999, Presented at 8th International Symposium on Field-Flow Fractionation FFF '99,
  • "Magnetic Nanoparticles: Past, Present and Future Applications in Biological Systems." 2003, Presented at Biochemical Engineering XIII,
  • "Examples of the Application of the Quadrupole Magnetic Cell Sorter: Rare Cell Selection and T-cell Depletion." 2003, Presented at 11th International Symposium on Field-Flow Fractionation,
  • "Electromagnetic Cell Tracking Velocimetry." 2003, Presented at AIChE Annual Meeting,
  • "Characterization of Magnetic Nano-Carriers." 2003, Presented at AIChE Annual Meeting,
  • "Physiological Effects and Potential Inhibition of Hydrodynamic Forces on Animal Cells." 2004, Presented at Cell Culture Engineering IX,
  • "Scale-up of Animal Cell Bioprocesses Equipment: Overview and Current Unknowns." 2004, Presented at Pfizer,
  • "Two Circuits or One? Should O2 addition and CO2 removal be uncoupled?." 2004, Presented at AIChE Annual Meeting,
  • "Exploitation of Magnetic Nano-particles in Cell Analysis and Separation: Theoretical, Experimental and Clinical Considerations." 2005, Presented at UMBC,
  • "Mass screening of surfactants Additives for Mammalian Cell Culture." 2006, Presented at ACS Annual Meeting,
  • "Biocompatible and Biodegradable Nanoparticle Labels." 2006, Presented at AIChE Annual meeting,
  • "Optimization of alloreactive depleted T-cell transplants: The use of MACS beads and MACS columns." 2007, Presented at AICHE Annual Meeting,
  • "Quantification of Nonspecific Binding of Nanoparticles using cell tracking velocimetry: Implication for detection and magnetic cell separation." 2009, Presented at ACS Meeting,
  • "Does EMT occur in circulating tumor cells of head and neck cancer patients?." 2010, Presented at American Association for Cancer Research: EMT and Cancer Progression and Treatment,
  • "Significance of circulating tumor cells in patients with squamous cell carcinoma of the head and neck." 2010, Presented at AHNS,
  • "Blood Pharming: Infinite Supply of fresh RBCs?." 2010, Presented at Cell Culture Engineering XII,
  • "Enrichment of CTCs in clinical samples using purely negative selection: Current status and potential." 2010, Presented at ADAPT 2010,
  • "Mutliparameter Analysis of negatively enriched, circulating tumor cells, TCT, in head and neck cancer patients." 2010, Presented at 2010 ASCO-NCI-EORTC Annual Meeting on Molecular Markers in Cancer,
  • "Cell-Bubble Interactions: Proposed Mechanisms of Cell death in Sparged Bioreactors." 1991, Presented at Amgen,
  • "Where is the Engineering in Biochemical Engineering." 1993, Presented at Engineering Foundation Conference,
  • "Fluids Engineering of Bioprocess Equipment." 1997, Presented at ASME Bioprocess Equipment Seminar,
  • "Fluids Engineering of Bioprocess Equipment." 2000, Presented at ASME Bioprocess Equipment Seminar,
  • "Scale-up of Animal Cell Bioreactors." 2000, Presented at MERCK,
  • "Quantification of Lethal and NonLethal Effects of Hydrodynamic Forces on Animal Cells." 2002, Presented at AIChE Annual Meeting,
  • "active T-Cell Depletion: The solution to the challenge of bone marrow transplants." 2004, Presented at Cell Culture Engineering IX,
  • "Alloreactive T cell depletion using Quadrupole magnetic flow sorter for allogeneic stem cell transplantation and cellular therapy." 2004, Presented at AIChE Annual Meeting,
  • "Nanobeads for Cancer Cell Selection." 2005, Presented at Sixth Principal Investigators Meeting Innovative Molecular Analysis Technologies Program,
  • "Quantification of the field interaction parameter and the binding constants of several antibody-magnetic nanoparticle conjugates." 2005, Presented at Pacific Polymer Federation IX Conference,
  • "Relationship of Hydrodynamic Forces to Suspended Cells and Application of Magnetic Cell Separation Technology." 2005, Presented at Pfizer,
  • "Is it Del B, or Del B^2? That is the question." 2006, Presented at AIChE Annual meeting,
  • "Enrichment of CTCs in clinical samples using purely negative selection: Current status and potential." 2010, Presented at Biomarker Assay Development Conference,
  • "Unspecified." 2010, Presented at ACS National Meeting,
  • "Circulating Tumor Cells: Clinical Potential and Bioprocess considerations to develop a commercially viable process." 2010, Presented at University of Oklahoma,
  • "Magnetically Facilitated Cell Separation." 1994, Presented at Cell Culture Engineering IV, Engineering Foundation Conference,
  • "The Impact of Interfaces on Cells and Proteins." 1994, Presented at ASME Bioprocess Equipment Seminar,
  • "Determination of Cell Surface Marker Density Using Particle Tracking Velocity." 1997, Presented at Cleveland Clinic Foundation,
  • "A proposed methodology for Scale-Up and Scale-down of animal cell culture Bioreactors." 2000, Presented at Cell Culture Engineering VII,
  • "Exploitation of Immunomagnetic Labeling: Rapid Cell Sorting and Fractionation." 2000, Presented at Department of Chemical Engineering,
  • "Immunomagnetic Cell Separation: Experimental & Theoretical Characterization of Magnetophoretic Mobility." 2000, Presented at AIChE Annual Meeting,
  • "Characterization of the Magnetophoretic Mobility of cells Labeled with Paramagnetic Nanoparticels." 2001, Presented at Particles 2001,
  • "Scale-up of Mammalian Cell Bioreactors." 2002, Presented at American Association of Pharmaceutical Scientists National Biotechnology Conference,
  • "Development of a Reliable, Routine Process to Isolate/Enrich Cancer Cells from Bone and Peripheral Blood Samples." 2002, Presented at 2nd International NCI-EORTC Meeting on Cancer Diagnostics,
  • "Fluids Engineering of Bioprocess Equipment." 2002, Presented at ASME Bioprocess Equipment Seminar,
  • "An attempt to Develop a Mechanistic Methodology to Understand Interfacial Phenomena in Animal Cell Culture." 2003, Presented at AIChE Annual Meeting,
  • "Unspecified." 2003, Presented at AIChE Annual Meeting,
  • "Screening of protein library by magnetic separation: a theoretical analysis." 2004, Presented at 227th ACS Spring Meeting,
  • "Development of 2-D and 3-D culturing techniques to isolate Pia-Arachnoid Cells from human brain tissue." 2004, Presented at AIChE Annual Meeting,
  • "An In Vitro Model to Study Cerebrospinal Fluid Physiology and Pseudotumor Cerebri." 2004, Presented at AIChE Annual Meeting,
  • "Quantification of the field interaction parameter of magnetic nanoparticles and the effect of magnetic nanoparticles on antibody binding affinity." 2005, Presented at BioChem Eng XIV,
  • "Potential new protective additives and delivery methods for Cell Culture." 2008, Presented at Cell Culture Engineering XI,
  • "Isolation of circulating tumor cells (CTCs) with mesenchymal and stem cell markers in localized and metastatic breast cancer using a novel negative selection enrichment." 2010, Presented at American Association for Cancer Research,
  • "Process Scale-up Considerations for Therapeutic Antibody Production." 2010, Presented at Beijing International Healthcare Industry Forum,
  • "Theoretical and Experimental Verification of Changes in oxygen binding capability of Red Blood Cell based on magnetic susceptibility." 2010, Presented at AIChE Annual Meeting,
  • "Expanding the definition of traditional CTCs: Detection and characterization of cells associated with cancer in the blood of patients with solid tumors." 2011, Presented at Lorentz Center workshop: Circulating tumor cell isolation and diagnostics,
  • "bius Sensor Ready Technology: A Novel Approach to Monitoring Single-Use Bioreactors." 2011, Presented at ESCAT,
  • "Microscopic Visualizations of Cell-Bubble Interactions." 1990, Presented at Annual AIChE Meeting,
  • "Continuous Flow Magnetic Cell Sorting." 1996, Presented at ESAO 96,
  • "Characterization of Biodegradation of Organic Compounds on a Micron-Scale." 1996, Presented at Annual AIChE Meeting,
  • "Immunomagnetic Cell Separation." 1997, Presented at Biochemical Engineering X, Engineering Foundation Conferences,
  • "Issues Surrounding the Scale-up of Animal Cell Culture." 1998, Presented at Biogen Inc.,
  • "Exploitation of Immunomagnetic Labeling, Rapid Cell Sorting and Fractionation." 1999, Presented at Biochemical Engineering XI,
  • "Detection of disseminated tumor cells in bone marrow of breast cancer patients." 2006, Presented at 6th International Conference on the Scientific and Clinical Applications of Magnetic Carriers,
  • "Detection and Quantification of Low Expressing Surface Markers Using Magnetic Nanoparticles." 2007, Presented at BioChem Eng XV,
  • "Magnetic Sorting of Unlabeled Red Blood Cells (RBC)." 2009, Presented at AIChE Annual Meeting,
  • "Mutliparameter Analysis of circulating tumor cells, CTC, in clinical samples using purely negative selection." 2010, Presented at AICHE Annual Meeting,
  • "Process Scale-up Considerations for Therapeutic Antibody Production." 2011, Presented at Centocor R&D,
  • "Studies on the Continuous Production and Excretion of a Plasmid Encoded Protein from Escherichia coli." 1988, Presented at First Annual Ohio state Biotechnology Conference,
  • "Investigations into the Mechanisms of Cell Damage/Death in Sparged Bioreactors." 1990, Presented at DuPont,
  • "Unspecified." 1990, Presented at AIChE Annual Meeting,
  • "Cell-Bubble Interactions: Proposed Mechanisms of Cell death in Sparged Bioreactors." 1991, Presented at Engineering Foundation Conference,
  • "Quantification of Cell Damage as a Result of Bubble Rupture." 1992, Presented at Annual AIChE Meeting,
  • "Determination of the Mechanism of Non-Specific Cell Adhesion to Gas-Liquid Interfaces." 1992, Presented at Annual AIChE Meeting,
  • "Local Energy Dissipation and Shear Sensitive Cells in Bioreactors, and Interfacial Phenomena in Bioprocessing." 1996, Presented at Genentech,
  • "Fluids Engineering of Bioprocess Equipment." 1996, Presented at ASME Bioprocess Equipment Seminar,
  • "Characterization and Quantification of Microbial Activity During the Composting Process." 1996, Presented at World Environmental Congress (World '96),
  • "Local Energy Dissipation and Shear Sensitive Cells in Bioreactors." 1997, Presented at 213th ACS National Meeting,
  • "Fast cell sorting in a novel, flow-through quadrupole magnetic field separator." 1997, Presented at 10th Symposium Molecular Biology of Hematopoiesis and Treatment of Leukemia and Lymphomas,
  • "Fast Cell separation with immunomagnetic colloid and flow-through magnetic sorter." 1998, Presented at Cell Culture Engineering VI, Engineering Foundation Conference,
  • "Magnetic Flow Sorting." 1998, Presented at Scientific and Clinical Applications of Magnetic Carriers,
  • "Cell Tracking Velocity." 1998, Presented at Scientific and Clinical Applications of Magnetic Carriers,
  • "Quantification of Cellular Properties from External Fields and Resulting Induced Velocity: Cellular Hydrodynamic Diameter and Magnetic Susceptibility." 1998, Presented at 214th ACS National Meeting,
  • "Rapid Cell Isolation by Magnetic Flow Sorting Using Immunomagnetic Labels in the Nanometer Scale." 1998, Presented at Annual AIChE Meeting,
  • "Impact of Media Phase Microstructuring on Intercellular Interactions." 1998, Presented at Annual AIChE Meeting,
  • "Cell Separation Case Studies: Fundamental and Scale-Up." 2000, Presented at ASME Bioprocess Equipment Seminar,
  • "A Proposed Methodology for the Scale-up/down of Animal Cell Bioreactors." 2000, Presented at Biogen,
  • "An Integrated Approach to Optimizing Immunomagnetic Cell Separation using a Quadrupole Magnetic Flow Sorter." 2000, Presented at AIChE Annual Meeting,
  • "Surfactants in Animal Cell Culture." 2001, Presented at MERCK,
  • "Cell Separation." 2001, Presented at Genomic Sample Preparation conference,
  • "Characterization of Magnetophoretic Mobility: An Overview." 2001, Presented at Biochemical Engineering XII,
  • "Alloreactive T cell depletion using Quadrupole magnetic cell sorter for allogeneic stem cell transplantation and cellular therapy." 2004, Presented at BMES,
  • "Effect of pH on cell density during isopycnic separation of cells using percoll density gradient." 2004, Presented at AIChE Annual Meeting,
  • "Development and/or Optimization of a Process to Obtain Homogeneous Cell Populations from Human Tumors." 2004, Presented at AIChE Annual Meeting,
  • "The Impact of Fluid Mechanical Forces in Flow Cytometers on Cells." 2004, Presented at AIChE Annual Meeting,
  • "Effects of Hydrodynamic Stress on Suspended Mammalian Cells." 2004, Presented at AIChE Annual Meeting,
  • "Magnetic Cell Separation: Biomedical, Bioprocess, or Biochemical? Its all Chemical Engineering to me!,." 2005, Presented at Department of Chemical Engineering,
  • "The Use of Energy Dissipation Rate as a Parameter to assist in the evaluation, scale-up, and scale-down of Bioprocesses." 2006, Presented at Cell Culture Engineering X,
  • "Mammalian Cell Fluid Mechanics and Scale-up Considerations Application and Design of Bioprocessing Equipment." 2006, Presented at Society of Bioprocessing Professionals 2006 Bioprocessing Institute,
  • "Magnetic separation methods for detection and analysis of circulating tumor cells. Enrichment of CTCs in clinical samples using purely negative selection: Current status and potential." 2009, Presented at Circulating Tumor Cells: Emerging Technologies for Detection, Diagnosis, Prognosis, and Treatment,
  • "Enrichment of CTCs in clinical samples using purely negative selection: Current status and potential." 2009, Presented at Circulating Tumor Cells: Emerging Technologies for Detection, Diagnosis, Prognosis, and Treatment,
  • "Overview of Posters." 2010, Presented at 2010 ASCO-NCI-EORTC Annual Meeting on Molecular Markers in Cancer,
  • "Unspecified." 1992, Presented at AIChE,
  • "Hydrodynamics Within Bioreactors." 1993, Presented at Merck, Sharp, & Dohme Research Laboratories,
  • "Experimental determination of local hydrodynamic information in microcarrier culture spinner flasks." 1993, Presented at 3rd International Conference on Bioreactor & Bioprocess Fluid Dynamics,
  • "Biodegradation of Organic Materials in Solid Wastes." 1993, Presented at Annual AIChE Meeting,
  • "Analysis of Hydrodynamic Information Obtained from Computer Simulations of the Rupture of a Gas Bubble in Relation to Animal Cell Damage in Sparged Bioreactors." 1993, Presented at Annual AIChE Meeting,
  • "The Use of Three-Dimensional Particle Image Velocimetry (3D-PIV) to Characterize the Hydrodynamics Forces on Anchorage Dependent Mammalian Cells." 1993, Presented at Annual AIChE Meeting,
  • "What every Cell Culturist Needs to Know About Bubbles." 1994, Presented at Cell Culture Engineering IV, Engineering Foundation Conference,
  • "Comparison of the Hydrodynamics in a 500 ml Spinner Flask, a 2L Bioreactor, and a 20 L Bioreactor with Respect to Microcarrier Culture." 1994, Presented at Annual AIChE Meeting,
  • "Development of a Bench-Scale Bioreactor for Composting Study." 1994, Presented at AIChE Annual meeting,
  • "Determination of the Magnetic Susceptibility of Labeled Cells by Video Imaging for Applications to Magnetic Cell Sorting." 1994, Presented at Annual AIChE Meeting,
  • "Study of the Influence of Protective Additives on the Interfacial Tensions between the Cell and the Surroundings." 1994, Presented at AIChE Annual Meeting,
  • "Unspecified." 1994, Presented at AIChE Annual Meeting,
  • "Theoretical and Experimental Measurements of the Distribution of Cell Surface Markers Densities Using Immunomagnetic Colloids (Nanometer Scale)." 1998, Presented at Annual AIChE Meeting,
  • "Fluids Engineering of Bioprocess Equipment." 1998, Presented at ASME Bioprocess Equipment Seminar,
  • "Scale-up of animal cell culture bioreactors based on local energy dissipation." 1999, Presented at 217th ACS National Meeting,
  • "Unspecified." 1999, Presented at 217th ACS National Meeting,
  • "Novel methods of cell separation using molecular and nanometer-scale magnetic reagents." 1999, Presented at First NASA & NCI Workshop on Sensors for Bio-Molecular Signatures,
  • "Unspecified." 1999, Presented at BioChem XI,
  • "Does Cell Surface Marker Density (number) Indicate Cell Function?." 1999, Presented at AIChE Annual Meeting,
  • "Mechanistic Studies of Interfacial Phenomena in CHO Cell Cultures." 2000, Presented at Cell Culture Engineering VII,
  • "Quantification of the Magnitude and Distribution of the local energy dissipation in bioreactors." 2000, Presented at Cell Culture Engineering VII,
  • "Exploitation of Immunomagnetic Labeling: Rapid Cell Sorting and Fractionation." 2000, Presented at Department of Bioengineering,
  • "Quantification of magnetophoretic mobility with Cell Tracking Velocimetry: Current and potential applications." 2000, Presented at . 3rd Scientific and Clinical Applications of Magnetic Carriers,
  • "Fluids Engineering of Bioprocess Equipment." 2000, Presented at MERIAL,
  • "Fluids Engineering of Bioprocess Equipment." 2000, Presented at MERCK,
  • "Unspecified." 2000, Presented at 3rd Scientific and Clinical Applications of Magnetic Carriers,
  • "Immunomagnetic Cell Separation for Rare Cancer Cell Detection in Blood: Current Applications and Future Potential." 2000, Presented at INNOVATIVE MOLECULAR ANALYSIS TECHNOLOGIES PI MEETING,
  • "Immunomagnetic Cell Separation for Rare Cancer Cell Detection in Blood: Current Applications and Future Potential." 2001, Presented at INNOVATIVE MOLECULAR ANALYSIS TECHNOLOGIES PI MEETING,
  • "Analysis of Antigen Expression on Hematopoetic Progenitor Cells for Magnetic Cell Separation." 2001, Presented at AIChE Annual Meeting,
  • "Fractionation of Immunomagnetically Labeled Hematopoietic Progenitor Cells into Functionally Different SubfractionsFractionation of Immunomagnetically Labeled Hematopoietic Progenitor Cells into Functionally Different Subfractions." 2001, Presented at AIChE Annual Meeting,
  • "CD34 Antigen Expression on Hematopoetic Progenitor Cells is Related to Efficiency of Magnetic Cell Separation." 2002, Presented at Cell Culture Engineering VIII,
  • "Characterization/quantification of the factors involved in imparting a magnetophoretic mobility on cells and particles." 2002, Presented at 4th Scientific and Clinical Applications of Magnetic Carriers,
  • "Evaluation of the performance of a commerical immunomagnetic cell separation system and the “Fractionationâ€� of CD34+ cells based on magnetophoretic mobility." 2000, Presented at 3rd Scientific and Clinical Applications of Magnetic Carriers,
  • "Exploitation of Magnetic Nano-particles in Cell Analysis and Separation: Theoretical, Experimental and Clinical Considerations. Department of Material Science, Stanford University, May 23, 2005.." 2004, Presented at RPI,
  • "Quantification of the field interaction parameter and binding affinity of antibody or streptavidin/biotin conjugated magnetic nanoparticles." 2004, Presented at 5th International Conference on the Scientific and Clinical Applications of Magnetic Carriers,
  • "Alloreactive T-Cell Depletion: The solution to the challenge of bone marrow transplants?." 2004, Presented at 5th International Conference on the Scientific and Clinical Applications of Magnetic Carriers,
  • "Unspecified." 2004, Presented at 5th Scientific and Clinical Applications of Magnetic Carriers,
  • "Mammalian Cell Fluid Mechanics and Scale-up Considerations Application and Design of Bioprocessing Equipment." 2004, Presented at Society of Bioprocessing Professionals 2004 Bioprocessing Institute,
  • "Detection of Rare Cancer cells in Peripheral Blood." 2005, Presented at AIChE Annual meeting,
  • "The Scale-up of T Cell Depletion for Mismatched Bone Marrow Transplants." 2005, Presented at AIChE Annual meeting,
  • "Detection of Disseminated Tumor Cells in Bone Marrow of Breast Cancer Patients." 2005, Presented at AIChE Annual meeting,
  • "Generation of Alloreactive T cells In-Vitro for Cellular Therapy." 2005, Presented at AIChE Annual meeting,
  • "Microalgal Fermentation Scale-up Considerations." 2005, Presented at AIChE Annual meeting,
  • "The Effect of Magnetic Nanoparticles on the Binding Affinity/Avidity of Antibody-Antigen Interactions." 2005, Presented at AIChE Annual meeting,
  • "Mammalian Cell Fluid Mechanics and Scale-up Considerations Application and Design of Bioprocessing Equipment." 2007, Presented at Society of Bioprocessing Professionals 2006 Bioprocessing Institute,
  • "Shear Sensitivity: What’s all the Fuss?." 2004, Presented at Eli Lilly and Company,
  • "Effect of hydrodynamic conditions on cell cycle and stress proteins." 2008, Presented at AICHE Annual Meeting,
  • "Quantification of non-Specific Binding of Magnetic Nanoparticles: Implication for detection and magnetic cell separation." 2008, Presented at AICHE Annual Meeting,
  • "Are circulating tumor cells present in the peripheral blood of cancer patients cancer stem cells?." 2009, Presented at ACS Meeting,
  • "Design Considerations in Continuous Magnetic Cell Separation." 1995, Presented at Annual AIChE Meeting,
  • "Free-Flow Magnetic Cell Sorting in Axially-Symmetric Split Column." 1996, Presented at Flow Field Fractionation 96,
  • "Continuous Magnetic Cell Separation." 1996, Presented at Whitaker Foundation Conference,
  • "Characterization of the Hydrodynamics in Different Types and Sizes of Bioreactors." 1996, Presented at Genentech Inc,
  • "Panel Discussion on Gas-Liquid Interfaces and Cells." 1996, Presented at 42nd Annual Conference of American Society of Artificial Internal Organs,
  • "Local Energy Dissipation and Shear Sensitive Cells in Bioreactors, and Interfacial Phenomena in Bioprocessing." 1996, Presented at 10th International Biotechnology Symposium,
  • "Physics of Magnetic Cell Sorting." 1996, Presented at Scientific and Clinical Applications of Magnetic Carriers,
  • "Development of a Reliable, Routine Process to Isolate/Enrich Cancer Cells from Bone and Peripheral Blood Samples." 2002, Presented at INNOVATIVE MOLECULAR ANALYSIS TECHNOLOGIES PI MEETING,
  • "Development of a Reliable, Routine Process to Isolate/Enrich Cancer Cells from Bone and Peripheral Blood Samples." 2002, Presented at AIChE Annual Meeting,
  • "A Novel Immunomagnetic Based Technology to Quantify Antigen Binding Capacity (ABC) on Human Aveolar Macrophages." 2003, Presented at American Thorasic Society (ATS),
  • "Scale-up of Mammalian Cell Bioreactor and Bioprocess Equipment." 2003, Presented at Wythe Pharmaceuticals,
  • "Quantification of Lethal and Non-lethal Effects of Hydrodynamic forces on Animal Cells." 2003, Presented at Biochemical Engineering XIII,
  • "High-throughput Immunomagnetic Cell Separation." 2003, Presented at Biochemical Engineering XIII,
  • "Mammalian Cell Fluids Mechanics and Scale-up Considerations." 2003, Presented at SBP Institute,
  • "Immuno-Magnetic Separation of Bacillus Cereus Spores form Foods." 2003, Presented at 11th International Symposium on Field-Flow Fractionation,
  • "Transport Processes in Magnetic Axisymmetric SPLITT Fractionation." 2003, Presented at 11th International Symposium on Field-Flow Fractionation,
  • "Magnetic Contrast Agent Enhances Performance of MgSPLITT." 2003, Presented at 11th International Symposium on Field-Flow Fractionation,
  • "Solid Phase/Nanoparticle Binding Studies." 2003, Presented at AIChE Annual Meeting,
  • "Hydrodynamic Forces: Apoptosis and Cytoskeletal Effects." 2003, Presented at AIChE Annual Meeting,
  • "Development and/or Optimization of a Process to Obtain Homogenous Cell Populations from Human Tumors." 2003, Presented at AIChE Annual Meeting,
  • "Immunomagnetic Cell Separation: Application of the Quadrupole Cell Sorter (QMS) to T-cell Depletion for Allogeneic Transplantation." 2003, Presented at AIChE Annual Meeting,
  • "Unspecified." 2004, Presented at Cell Culture Engineering IX,
  • "Exploitation of Magnetic Nano-particles in Cell Analysis and Separation: Theoretical, Experimental and Clinical Considerations. Department of Material Science, Stanford University, May 23, 2005.." 2005, Presented at Department of Material Science,
  • "Quantification of the field interaction parameter and the binding constants of several antibody-magnetic nanoparticle conjugates." 2005, Presented at 3rd International Meeting on the Diagnostic and Biotechnology Applications of SuperParaMagnetic Microspheres,
  • "Detection of rare circulating/disseminated tumor cells in human body fluids." 2005, Presented at BioChem Eng XIV,
  • "Optimize the generation and depletion of alloreactive T cell subsets for cellular therapy." 2005, Presented at BioChem Eng XIV,
  • "Fractionation of Immunomagnetically Labeled, Human Cord Blood Progenitor Cells Using a Novel Dipole Magnetic Flow Cell Sorter." 2005, Presented at BioChem Eng XIV,
  • "The use of Energy Dissipation Rate as a parameter to assist in the evaluation , scale-up, and scale-down of bioprcoesses." 2006, Presented at ACS Annual Meeting,
  • "The Use of Energy Dissipation Rate as a Parameter to assist in the evaluation, scale-up, and scale-down of Bioprocesses." 2006, Presented at AMGEN,
  • "The Use of Energy Dissipation Rate as a Parameter to Assist in the Evaluation, Scale-up, and Scale-down of Bioprocesses." 2006, Presented at AIChE Annual meeting,
  • "The Use of Energy Dissipation Rate as a Parameter to assist in the evaluation, scale-up, and scale-down of Bioprocesses." 2008, Presented at Genzyme,
  • "Isolation of circulating tumor cells (CTCs) with mesenchymal and stem cell markers in localized and metastatic breast cancer using a novel negative selection enrichment." 2010, Presented at American Association for Cancer Research: EMT and Cancer Progression and Treatment,
  • "Correlations of both the presence of and oxidation state of Mn in Bacillus attrophaeus spores and it imparting of magnetic susceptibility to the spores." 2010, Presented at ACS Meeting,
  • "Multparameter Analysis of potential CTC’s." 2010, Presented at World Circulating Tumor Cell Summit,
  • "Fluids Engineering of Bioprocess Equipment." 2001, Presented at ASME Bioprocess Equipment Seminar,
  • "Unspecified." 2004, Presented at AIChE Annual Meeting,
  • "Unspecified." 2005, Presented at AIChE Annual Meeting,
  • "The Long Term Effect of Constant, Well-Defined Laminar Shear Stress on Suspended Cells." 1989, Presented at Annual AIChE Meeting,
  • "The Effect of Well Defined Laminar Shear Stress on Suspended Insect Cells and The Protective Effects of Methylcellulose Derived Compounds." 1989, Presented at Cell Culture Engineering II, Engineering Foundation,
  • "Cell-Bubble Interactions: Proposed Mechanisms of Cell death in Sparged Bioreactors." 1990, Presented at Eli Lilly and Company,
  • "Local Energy Dissipation and Shear Sensitive Cells in Bioreactors." 1997, Presented at Merck Inc,
  • "Characterization of Animal Cell Bioreactors with Respect to Local Energy Dissipation and a Proposed, Rational Scale-up/Scale-down Methodology." 2002, Presented at American Chemical Society,
  • "Scale-up of Animal Cell Bioprocesses Equipment: Overview and Current Unknowns." 2004, Presented at Martek Biotechnology,
  • "Quantitative intracellular magnetic nanoparticle uptake determination from cell magnetophoresis." 2006, Presented at ACS Annual Meeting,
  • "Immunomagnetic Cell Separation: Fundamentals, Current Issues, and Future Potentials." 2007, Presented at AICHE Annual Meeting,
  • "The Use of Magnetic Nanoparticles to Enrich for Rare, Circulating Tumor Cells in Head and Neck Cancer." 2008, Presented at Nanomedicine Summit,
  • "netic Flow Sorting Using a Model System of Human Lymphocytes and a colloidal magnetic label." 1996, Presented at ASAIO,
  • "Mammalian Cell Culture Fluid Mechanics: Scale-up Considerations." 2005, Presented at Society for Industrial Microbiology annual meeting,
  • "Quantification of the field interaction parameter and the binding constants of several antibody-magnetic nanoparticle conjugates." 2005, Presented at 79th ACS Colloid and Surface Science Symposium,
  • "Large area dye mixing studies using image analysis." 2010, Presented at AIChE Annual Meeting,
  • "Investigation of Stress Response Expression in Animal Cell Culture." 2010, Presented at AIChE Annual Meeting,
  • "Bioreactors for Continuous Production and Excretion of Plasmid-Encoded Protein from E. coli." 1986, Presented at Cornell Biotechnology Symposium,
  • "Microscopic Observations of Cells Subjected to Various Levels of Shear Stress." 1988, Presented at Annual AIChE Meeting,
  • "Analysis of Hydrodynamic Information Obtained from Computer Simulations of the Rupture of a Gas Bubble in Relation to Animal Cell Damage in Sparged Bioreactors." 1993, Presented at 3rd International Conference on Bioreactor & Bioprocess Fluid Dynamics,
  • "Foaming and Interfacial Phenomena in Microcarrier Suspensions." 1996, Presented at Annual AIChE Meeting,
  • "An Integrated Approach to Optimizing Breast Cancer Cell Separation by Quadrupole Magnetic Flow Sorter." 2000, Presented at 3rd Scientific and Clinical Applications of Magnetic Carriers,
  • "Exploitation of Magnetic Nano-particles in Cell Analysis and Separation: Theoretical, Experimental and Clinical Considerations. Department of Material Science, Stanford University, May 23, 2005.." 2001, Presented at Biochemical Engineering XII,
  • "Non-ideal flow in Magnetic Cell Devices." 2002, Presented at AIChE Annual Meeting,
  • "Cell Magnetophoresis: Separation of erythroid cells based on hemoglobin magnetic susceptibility." 2002, Presented at 2002 Annual Meeting of the American Society of Hematology,
  • "Unspecified." 2005, Presented at BioChem Eng XIV,
  • "Enrichment of CTCs in clinical samples using purely negative selection: Current status and potential." 2009, Presented at ADAPT 2009,
  • "Cell Interactions with Rupturing Gas Bubbles." 1991, Presented at Annual AIChE Meeting,
  • "Mechanisms of Cell Damage as a Result of Sparging and Rational Criteria for Designing Medium to Prevent Damage." 1992, Presented at Engineering Foundation Conferences, Cell Culture Engineering III,
  • "Insect Cell Interactions with Gas Bubbles." 1992, Presented at Baculoviruses and Recombinant Protein Production Processes Workshop,
  • "Effect of Gas Sparging on Microcarrier Animal Cell Cultures." 1995, Presented at Chemical Society National Meeting,
  • "Quantification of Biodegradation using FTIR." 1995, Presented at AIChE Annual Meeting,
  • "Fluids Engineering of Bioprocess Equipment." 1995, Presented at ASME Bioprocess Equipment Seminar,
  • "Characterization of the Hydrodynamics in Different Types and Sizes of Bioreactors." 1995, Presented at 8th Annual Meeting of Japanese Association for Animal Cell Technology,
  • "Performance of a quadrupole-field flow cell sorter predicted on the basis of SPLITT formalism." 1999, Presented at 8th International Symposium on Field-Flow Fractionation FFF '99,
  • "Quantification of the Number of Cell Surface Markers (Receptors)." 1999, Presented at AIChE Annual Meeting,
  • "Can “Local Energy Dissipationâ€� be used to Quantify the Effect of Hydrodynamic Forces on Cells in Bioreactors and for Scale-up?." 1999, Presented at AIChE Annual Meeting,
  • "Fluids Engineering of Bioprocess Equipment." 1999, Presented at ASME Bioprocess Equipment Seminar,
  • "Magnetic-based Technologies." 2002, Presented at Detection and Measurement of Occult Disease for the Prognosis of Solid Tumors,
  • "Exploitation of Magnetic Nano-particles in Cell Analysis and Separation: Theoretical, Experimental and Clinical Considerations." 2003, Presented at Tufts University,
  • "Exploitation of Magnetic Nano-particles in Cell Analysis and Separation: Theoretical, Experimental and Clinical Considerations." 2004, Presented at FABE,
  • "Studies on the enrichment of rare cancer cells in human peripheral blood." 2004, Presented at BMES,
  • "Development &/or Optimization of a Process to Obtain Homogeneous Cell Populations from Human Tumors." 2004, Presented at BMES,
  • "Separation Technique to Isolate Arachnoid Cap Cells." 2004, Presented at BMES,
  • "Development of 2D & 3D culture techniques to isolate Pia-Arachnoid(PA) Cells from human brain tissue." 2004, Presented at BMES,
  • "Studies on the enrichment of rare cancer cells." 2004, Presented at AIChE Annual Meeting,
  • "Magnetic Detection of Spores." 2005, Presented at AIChE Annual meeting,
  • "The effect of multiple passes of EDR on suspended Animal Cells." 2005, Presented at AIChE Annual meeting,
  • "Magnetic Cell Separation: Biomedical, Bioprocess, or Biochemical? Its all Chemical Engineering to me!,." 2005, Presented at AIChE meeting,
  • "Comparison of Force Models in Predicting Performance of the Quadrupole Magnetic Sorter." 2006, Presented at 6th International Conference on the Scientific and Clinical Applications of Magnetic Carriers,
  • "Application of immunomagnetic cell enrichment in combination with RT-PCR for the detection of rare circulating cancer cells in peripheral blood and bone marrow from patients with Head and Neck Squamous Cell Carcinoma (HNSCC)." 2006, Presented at AIChE Annual meeting,
  • "New Immunomagnetic Beads for T cell depletion." 2006, Presented at AIChE Annual meeting,
  • "Relationship between Hydrodynamic Forces and the Cell Cycle: Implications to Bioprocessing." 2007, Presented at BioChem Eng XV,
  • "Detection of CTC in head and neck cancer patients with potential clinical outcomes." 2008, Presented at ASCO-NCI EORTC Annual Meeting on Molecular Markers in Cancer,
  • "Quantitative Study of Physiological Responses of CHO Cells to Repetitive Hydrodynamic Stress." 2008, Presented at AICHE Annual Meeting,
  • "Quadrupole magnetic SPLITT fractionation." 2009, Presented at ACS Meeting,
  • "Enrichment of CTCs in clinical samples using purely negative selection: Current status and potential." 2010, Presented at Genitourinary Oncology Service,
  • "Separating Magnetically labeled and Unlabeled Biological Cells within microfluidic channels using magnetic traps created with nanowires and disks." 2011, Presented at Biochemical and Molecular Engineering XVII: Emerging Frontiers,

Papers in Proceedings

2009

  • Chalmers,Jeffrey,J; Lang,James; Balasubramanian,P; Jatana,K; Schuller,D; Agrawal,A; Zborowski,Maciej "BIOT 22-Are circulating tumor cells present in the peripheral blood of cancer patients' cancer stem cells?." in ACS Meeting. (8 2009).

2005

  • Carpino F, Moore LR, Chalmers JJ, Zborowski M, Williams PS. "Quadrupole magnetic field-flow fractionation for the analysis of magnetic nanoparticles." in ICFPM Proceedings in Journal of Physics: Conference Series. (1 2005).

2004

  • Chalmers, J.J., Garcia-Briones, M., Bavarian, F., Hink, F. "Insect Cell Interactions with Gas Bubbles." (1 2004).

1999

  • Moore, L.R., Zborowski, M., Sun, Liping, Chalmers, J.J. "Lymphocyte Fractionation in a Magnetic Flow Cell Sorter." (1 1999).

1996

  • Zborowski, M., Moore, L.R., Reddy, S., Chen, G.H., Sun, L., Chalmers, J. "netic Flow Sorting Using a Model System of Human Lymphocytes and a colloidal magnetic label." in Unspecified. (1 1996).

1993

  • Venkat, R.V., Brodkey, R.S., Guezennec, Y.G., Chalmers, J.J. "Experimental determination of local hydrodynamic information in microcarrier culture spinner flasks." (9 1993).
  • Garcia-Briones, M.A., Chalmers, J.J. "Analysis of Hydrodynamic Information Obtained from Computer Simulations of the Rupture of a Gas Bubble in Relation to Animal Cell Damage in Sparged Bioreactors." in 3rd International Conference on Bioreactor & Bioprocess Fluid Dynamics. (9 1993).

1985

  • Arnold, F.H., Chalmers, J.J., Saunders, M.S., Croughan, M.S., Blanch, H.W., Wilke, C.R., "A Rational Approach to the Scale-up of Affinity Chromatography." (1 1985).