Wood Laboratory for Applied Protein Engineering

Biotechnology development through protein engineering

ABOUT US

Led by Professor David W. Wood, our work seeks to develop highly useful biotechnologies through engineering proteins for specific applications. So far, these applications include new ways to purify recombinant proteins, bacterial biosensors that incorporate human drug targets, and new capabilities in drug discovery and drug delivery.

  • Learn more about the Applied Protein Engineering Group in the video at the left.
  • To view in full-screen mode, click on the Play button and then on the square in the bottom right of the video.
  • Professor Wood is currently accepting students.

RESEARCH

The Applied Protein Engineering Group's research focus is on biotechnology development through protein engineering. Professor Wood is known for groundbreaking research in self-cleaving affinity tag technology for the purification of recombinant proteins. Current applications include new ways to purify recombinant proteins, bacterial biosensors that incorporate human drug targets, and new capabilities in drug discovery and drug delivery.

Since the mid-1980s, the use of affinity tag technology has been a ubiquitous method for laboratories to purify virtually any arbitrary recombinant protein through a single general and simple method. While dozens of different tags, kits and accessories are now commercially available, this method has not been adopted for large-scale bioprocessing, largely due to the expense of removing the affinity tag after the target protein is purified. 

The group is currently developing new protein elements that effectively make the affinity tags self-cleaving after purification, therefore enabling many new tag-based protein purification methods that address the biotech industry's needs for scale, simplicity and cost control.

Prospective students, postdoctoral researchers, and visiting scholars should check out our lab group members' personal pages to see individual projects that are currently active in pursuit of our research goals.

Gonzalez-Serrano, Sabat

Sabat Gonzalez-Serrano

Doctoral program

Marshall, Brian A.

Brian A. Marshall

Doctoral program

Moody, Nathan A.

Nathan M. Moody

Masters program

Prabhala, Sai Vivkek

Sai Vivek Prabhala

Doctoral program

Yuan, Hongyu

Hongyu Yuan

Doctoral program

News from the Lab

FACILITIES and EQUIPMENT

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Lab facilities are located in the CBEC building, which was constructed in 2015 with state-of-the-art features.

Wood Lab has a wide variety of equipment facilitating biopharmaceutical research.  Interested students, visiting scholars and collaborators should contact Professor David Wood at wood.750@osu.edu and visit the Chemical and Biomolecular Engineering homepage.

Industrial and private clients are likewise encouraged to contact Professor David Wood to see how our lab can develop solutions to address your bioprocessing needs.

akta

AKTA fast protein liquid tomography

An AKTA pure and AKTA purifier model allow automation of protein chromatography processes.

Added columns can extensively monitor the purification process and do kinetics experiments.

biophotometer

Biophotometer

Our Eppendorf Biophotometer facilitates DNA and protein quantitation as well as cell density measurements.

Wood Lab-Bioreactor

Fermenter

Our New Brunswick Scientific BIOFLO 110 Fermenter / bioreactor is used for large-scale (up to 5L) protein expressions and fermentation experiments.

gel imager

Gel imaging system

A gel imaging system can capture images of DNA and Protein gels.

liquid handling robots

Liquid handling robots

Two Beckman laboratory automation workstations greatly facilitate high-throughput screening experiments.

Used primarily by the biosensor sub-group and other projects performing directed evolution experiments.

microplate

Multi-mode microplate reader

A BioTek Multi-Mode Microplate Reader enables high- and medium-throughput screening experiments for our biosensors and yeast display projects.