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David Wood

  • Professor, Chemical & Biomolecular Eng
  • 456 CBEC
    151 W. Woodruff Ave.
    Columbus, OH 43210
  • 614-292-9636

About

Education

  • B.S., Biology and Chemical Engineering, California Institute of Technology, 1990 
  • M.S., Chemical Engineering, Rensselaer Polytechnic Institute, 1997 
  • Ph.D., Rensselaer Polytechnic Institute, 1999

 

Key Honors and Distinctions

  • Known for groundbreaking research in self-cleaving affinity tag technology for the purification of recombinant proteins.

Top Awards

  • Lumley Research Award, 2016, The Ohio State University College of Engineering
  • NSF CAREER Award, 2003

RESEARCH AREAS - Wood Lab for Applied Protein Engineering

  • Protein engineering for bioseparations, biosensing and drug discovery.
  • Graduate student research opportunities are currently available.
  • PUBLICATIONS AND PATENTS

Applied protein engineering for biotechnology development
My work seeks to develop highly useful biotechnologies through engineering proteins and enzymes for specific applications.  So far, these applications include new ways to purify recombinant proteins, bacterial biosensors that incorporate human drug targets, and new capabilities in drug discovery and drug delivery.

Bioseparations
The development of affinity tag technology in the mid 1980's provided a means to purify virtually any arbitrary recombinant protein through a single general and simple method.  This method is now ubiquitous in the laboratory, where dozens of different tags, kits and accessories are now commercially available.  Surprisingly, this method has not been adopted for large-scale bioprocessing, largely due to the expense of removing the affinity tag after the target protein is purified.  Thus the promise of this method has been severely limited due to basic limitations in the proteins involved.

We are currently developing new protein elements that effectively make the affinity tags self-cleaving after purification, and therefore enable many new tag-based protein purification methods.  These methods retain the simplicity and generality of conventional affinity tag technology, and can potentially make tag technology a ubiquitous and critical platform in protein bioseparations.  Although our early methods are very effective for proteins expressed in E. coli, we must adapt this technology for proteins expressed in Pichia and CHO, as well as transgenic and other expression hosts.  Further, the tags we use must be attractive to the biotech industry, and address its needs for scale, simplicity and expense. 

Thus our focus is on the development of new tags that meet these needs.  For example, these tags must bind at high capacity, and have chemistries that lend themselves to disposable technologies, and the self-cleaving reaction must be more controllable and reliable in every expression host.  Our approaches range from simple chemistry to complex protein re-design, and require a variety of backgrounds.

Biosensing and Drug Discovery
The nuclear hormone receptors (NHRs) are involved in vital functions of the cell, such as development, differentiation, homeostasis, reproduction and metabolism.  Accordingly, NHRs comprise one of the most important classes of drug targets, and about 4% of all currently marketed therapeutics interfere with the activity of one or more of these proteins.  Importantly, they are also targets for naturally occurring and synthetic endocrine disrupting compounds, and interference with their function has been connected to breast cancer, decreased fertility, and other disorders in humans and in animals.

We have developed a very simple reporter system in E. coli that can sense human hormone-like compounds, and report their presence and activity through changes in growth phenotype.  Our sensor is based on a chimeric fusion protein where genetically inserting various hormone ligand-binding domains yields sensors with altered specificity.  We have now constructed sensors using the alpha and beta subtypes of the human estrogen receptor, as well as the human thyroid receptor and insect ecdysone receptor.  These designs are not only able to detect hormone-like compounds for these targets, but they are also able to discriminate between estrogen agonistic and antagonistic activities, distinguish subtype-selective estrogen receptor modulators, and report rough estimates of binding affinity.  Our current goal is to understand the structure, energy and kinetics of our designed proteins, and generate new rules for engineering allosteric protein chimeras.  In the short term we hope to modify this system for screening large natural-product libraries, and libraries derived from combinatorial synthesis techniques.  The ability of this system to work effectively with three examples of human nuclear hormone receptors suggests that it will be generally useful for identifying natural product ligands that modulate this family or targets.

Honors

  • 20030101

    Career Award.

Presentations

  • "Precise Control of Effluent Concentration and Peak Shape through the Use of Self-Cleaving Affinity Tags in Protein Affinity Separations." 2004, Presented at AIChE National Meeting,
  • "Elastin-Like-Polypeptide And Polyhydroxybutyrate-Intein Mediated Protein Purification." 2007, Presented at AIChE National Meeting,
  • "Recombinant Protein Purification Using Self-Cleaving Non-Chromatographic Bioseparation Tags." 2009, Presented at PepTalk: Processes to Optimize Protein Purification and Recovery,
  • "Simple and Economical Purification of Recombinant Proteins by Self-Cleaving Aggregation Tags." 2006, Presented at AIChE National Meeting,
  • "Self-Associating Self-Cleaving Tags for Protein Purification." 2007, Presented at Accelerating BioPharmaceutical Development,
  • "Bacterial biosensors: Design and application." 2010, Presented at The 239th ACS National Meeting,
  • "Engineered Self-Cleaving Purification Tags for Rapid Cloning and Protein Purification." 2009, Presented at AIChE National Meeting,
  • "Sequence optimization of elastin-like polypeptide and its potential use in non-chromatographic bioseperation." 2010, Presented at The 239th ACS National Meeting,
  • "Modulating the Sensitivity of a Bacterial Biosensor for Peroxisome Proliferator-Activated Receptors Gamma to the Ligands by Engineering Spacer Linker." 2010, Presented at AIChE National Meeting,
  • "Generating Small-Molecule-Controlled Inteins for Applications in Biotechnology." 2003, Presented at Mid Atlantic Bioengineering Consortium Conference,
  • "A Novel, Universal Protein Purification Technology: One-Step Affinity Purification Using Simple Centrifugation." 2003, Presented at AIChE National Meeting,
  • "Construction of Simple In Vivo Drug Screening Systems." 2005, Presented at Mid Atlantic Bioengineering Consortium Conference,
  • "Engineered In Vivo Biosensors for Nuclear Hormone Receptor Ligands." 2005, Presented at Biochemical Engineering XIV,
  • "New Bioseparations Techniques Using Self-Cleaving Affinity Tags." 2005, Presented at Biochemical Engineering XIV,
  • "Novel biosensor for thyroid hormone endocrine disruptors." 2008, Presented at The 23086th ACS National Meeting,
  • "Simple, Effective Bioseparations Using Self-Cleaving Purification Tags." 2006, Presented at Recovery Of Biological Products XII,
  • "Engineering Allosteric Inteins." 2006, Presented at Mid Atlantic Bioengineering Consortium Conference,
  • "Self-cleaving Tags for Non-chromatographic Capture and Purification of Recombinant Proteins." 2008, Presented at Antibody Development & Production,
  • "Self-Cleaving Affinity Tags Re-Engineered for High-Throughput Applications." 2004, Presented at The 227th ACS National Meeting,
  • "Engineering Hormone-Sensitive Bacteria: A Step Further Towards Efficient Drug Screening." 2004, Presented at Mid Atlantic Bioengineering Consortium Conference,
  • "Engineering Hormone-Sensitive Bacteria for Efficient Drug Screening." 2004, Presented at AIChE National Meeting,
  • "Controlling Protein Function with Peptide Splicing." 2004, Presented at AIChE National Meeting,
  • "Self-Cleaving Affinity Tags Re-Engineered for High-throughput Applications." 2004, Presented at AIChE National Meeting,
  • "A Optimized Chimeric Estrogen-Sensor Protein: Applications in Drug Discovery and Biosensing." 2006, Presented at AIChE National Meeting,
  • "Probing Into the Mechanism for Bacterial Biosensor for Nuclear Receptor Ligand." 2010, Presented at AIChE National Meeting,
  • "Advances in Non-Chromatographic Bio-Separation Techniques; Significantly Improving Process Through-Out' Production." 2009, Presented at Biolnnovation Leaders Summit,
  • "Self-cleaving Fusion Tags for the Non-chromatographic Capture and Purification of Recombinant Proteins." 2008, Presented at BioProcess International,
  • "A Novel Screening/Selection system for Protein Splicing." 2003, Presented at AIChE National Meeting,
  • "Generating Small-molecule-controlled Inteins for Applications in Biotechnology." 2003, Presented at AIChE National Meeting,
  • "Statistical Analysis of Evolutionary Patterns for Protein Engineering." 2003, Presented at AIChE National Meeting,
  • "A Novel Protein Purification Technology: One-Step Affinity Purification Using Simple Centrifugation." 2004, Presented at Mid Atlantic Bioengineering Consortium Conference,
  • "An Engineered Chimeric Enzyme for the Detection and Discovery of Estrogen-Like Compounds." 2006, Presented at The 20th Symposium of the Protein Society,
  • "Non-Chromatographic Bioseparations Using Self-Cleaving Aggregation Tags." 2006, Presented at The 232nd ACS National Meeting,
  • "Engineered Allosteric Proteins for Sensing and Classifying Estrogenic Compounds." 2006, Presented at The 232nd ACS National Meeting,
  • "Recent developments in intein-mediated protein purification technology." 2010, Presented at The 239th ACS National Meeting,
  • "Emerging Alternatives to Conventional Downstream Technologies: Considerations and Implementation at Preclinical Scale." 2010, Presented at Preclinical Scale Bioprocessing,
  • "Application of Self-Cleaving Binding Domains to Affinity Separations." 2001, Presented at Recovery of Biological Products X,
  • "Self-cleaving intein-mediated protein purification in Pichia pastoris." 2008, Presented at The 236th ACS National Meeting,
  • "Concentration-Controlled Single-Step Protein Purification Using a Tunable Self-Cleaving Affinity-Tag." 2009, Presented at AIChE National Meeting,
  • "Reducing the Cost of Affinity Purification: In Vivo Affinity Matrix Production and Application of an Engineered Self-Cleaving Intein." 2005, Presented at The 229th ACS National Meeting,
  • "Novel Technology for Recombinant Protein Purification: One-Step Affinity Purification Using Simple Centrifugation." 2005, Presented at The 229th ACS National Meeting,
  • "Application of the ELP-intein-based nonchromatographic protein purification technique to the CHO expression system." 2008, Presented at The 236th ACS National Meeting,
  • "Purification of assembled multisubunit Escherichia coli RNA polymerase by a self-cleaving elastin-like polypeptide (ELP) tag." 2009, Presented at The 238th ACS National Meeting,
  • "Investigation of Hofmeister ions to optimize ELP-intein mediated protein purification." 2009, Presented at The 238th ACS National Meeting,
  • "Engineered Allosteric Proteins for Drug Discovery and Environmental Monitoring." 2011, Presented at International Conference on Biomolecular Engineering (ISBE),
  • "Self-Cleaving Tags: New Options and New Applications." 2011, Presented at PepTalk: Protein Purification and Recovery: Tag Technologies,
  • "New Technologies From Genetically Engineered Inteins: From Hormone Sensing to Non-Chromatographic Bioseparations." 2007, Presented at Wadsworth Center of the New York State Department of Health Departmental Seminar,
  • "Engineering Allosteric Inteins For Use As Biosensors." 2007, Presented at AIChE National Meeting,
  • "Self-Cleaving Tags Based on Engineered Inteins: Recent Results and Future Prospects." 2009, Presented at The Bioprocessing Summit: Affinity Tag Protein Purification,
  • "Waste (and Cost) Reduction through Non-chromatographic Purification Platform Technologies." 2010, Presented at GREENbiopharma,
  • "Next-generation inteins for applications in mammalian cells." 2012, Presented at Non-Antibody Protein Production,
  • "An effective self-cleaving tag platform for proteins expressed in mammalian cells." 2012, Presented at Recovery VX,
  • "A Self-Cleaving Purification Tag System For Use In Mammalian Cell Culture." 2012, Presented at The Bioprocessing Summit: Overcoming Purification Challenges,
  • "New Technologies from Self-Modifying Proteins." 2003, Presented at Merck Bioprocess Symposium,
  • "An Engineered Self-Cleaving Tag System for Use with Any Expression Host." 2013, Presented at PepTalk: Protein Purification and Recovery: Streamlining Processes,
  • "Tag alternatives to Protein A: Is it time?." 2014, Presented at BioProduction 2014,
  • "Creating and purifying ANY therapeutic protein in a portable bioreactor: A tangible option for the future of drug manufacture?." 2015, Presented at Cell Culture World and Downstream Processing World Conference 2015,
  • "Self-cleaving Non-chromatographic Purification Tags: Current Results and Future Prospects." 2011, Presented at BioProcess International,
  • "Tag Technologies: Opportunities and Challenges in the Biopharmaceutical Industry." 2011, Presented at The Bioprocessing Summit: Affinity Tag Protein Purification,
  • "Establish a Successful Purification Platform." 2012, Presented at Non-Antibody Protein Production,
  • "Engineering New Inteins for Better Bioseparations." 2001, Presented at Promega Corporation Corporate Seminar,
  • "New Technologies from Self-Modifying Proteins." 2006, Presented at University of Pennsylvania Departmental Seminar,
  • "New Technologies from Self-Modifying Proteins." 2006, Presented at Bristol Meyers Squibb Corporate Seminar,
  • "New Technologies from Self-Modifying Proteins." 2006, Presented at The Cleveland Clinic Foundation Departmental Seminar,
  • "New Technologies from Self-Modifying Proteins." 2006, Presented at Rensselaer Polytechnic Institute Departmental Seminar,
  • "New Technologies from Self-Modifying Proteins." 2006, Presented at North Carolina State University Departmental Seminar,
  • "New Technologies from Self-Modifying Proteins." 2007, Presented at Clarkson University Departmental Seminar,
  • "Novel Bioseparations Using Self-Cleaving Purification Tags." 2009, Presented at Corporate Seminar,
  • "Ligand-Induced Protein Cleavage for Biosensor Applications." 2002, Presented at Lifesensors Inc. Corporate Seminar,
  • "New Technologies from Self-Modifying Proteins." 2005, Presented at Johns Hopkins University Departmental Seminar,
  • "New Technologies from Self-Modifying Proteins." 2005, Presented at Columbia University Departmental Seminar,
  • "New Technologies from Engineered Self-Modifying Proteins." 2013, Presented at Michigan Technological University Departmental Seminar,
  • "Application of Self-Cleaving Binding Domains to Affinity Separations." 2001, Presented at Glaxo Smith Kline Corporation Corporate Seminar,
  • "Modifying Inteins for Use in the Gateway Cloning System." 2001, Presented at Invitrogen Corporation Corporate Seminar,
  • "Searching for Faculty Positions." 2002, Presented at Rensselaer Polytechnic Institute Departmental Seminar,
  • "Application of Self-Cleaving Binding Domains to Affinity Separations." 2002, Presented at DGI Biotechnologies Corporate Seminar,
  • "Simple Protein Purification Using Self-Cleaving Aggregation Tags." 2005, Presented at Amgen, Inc. Corporate Seminar,
  • "New Technologies from Self-Modifying Proteins." 2007, Presented at Cambridge University Departmental Seminar,
  • "Simple Biosensors to Detect Endocrine Active Compounds: Application to ASD Targets." 2009, Presented at Nancy Lurie Marks Family Foundation Boston Club Presentations,
  • "New Technologies from Self-Modifying Proteins." 2007, Presented at Heriot-Watt University Departmental Seminar,
  • "Single-Step Protein Purification Using Controllable Self-Cleaving Affinity Tags." 2001, Presented at Dartmouth College Departmental Seminar,
  • "New Technologies from Self-Modifying Proteins." 2006, Presented at Cornell University Departmental Seminar,
  • "New Technologies from Self-Modifying Proteins." 2006, Presented at Tulane University Departmental Seminar,
  • "Spinning New Biotechnologies out of Genetic Junk." 2010, Presented at Chicago State University Departmental Seminar,
  • "New Technologies from Engineered Self-Modifying Proteins." 2010, Presented at SUNY Buffalo Departmental Seminar,
  • "A Self-Cleaving Purification Tag System For Use In Mammalian Cell Culture." 2012, Presented at Genentech Corporate Seminar,
  • "New Technologies from Self-Modifying Proteins." 2007, Presented at Oxford University Departmental Seminar,
  • "New Technologies from Engineered Self-Modifying Proteins." 2011, Presented at Polytechnic University of New York Departmental Seminar,
  • "Simple Protein Purification Using Self-Cleaving Aggregation Tags." 2006, Presented at Bio-Rad Incorporated Corporate Seminar,
  • "High Throughput Screening of Human Hormone-Like Compounds." 2006, Presented at Pharmacopeia Drug Discovery Inc. Corporate Seminar,
  • "New Technologies from Self-Modifying Proteins." 2007, Presented at University of Maryland at College Park Departmental Seminar,
  • "Novel Bioseparations Using Self-Cleaving Purification Tags." 2009, Presented at National Cancer Institute at Frederick, SAIC Corporate Seminar,
  • "New Technologies from Self-Modifying Proteins." 2009, Presented at City College of New York AIChE Ambassador Series,
  • "Engineered Bacteria for Sensing Hormone-like Chemicals: Applications from Drug Discovery to Environmental Monitoring." 2015, Presented at Kenyon College Departmental Seminar,
  • "New Technologies from Self-Modifying Proteins." 2005, Presented at University of Maryland at Baltimore County Departmental Seminar,
  • "New Technologies from Self-Modifying Proteins." 2007, Presented at University of Houston Departmental Seminar,
  • "New Technologies from Self-Modifying Proteins." 2009, Presented at Rensselaer Polytechnic Institute Departmental Seminar,
  • "New Technologies from Self-Modifying Proteins." 2007, Presented at University of Birmingham, U.K., Departmental Seminar,
  • "New Technologies from Self-Modifying Proteins." 2007, Presented at Biogen Idec, Inc., Bioprocess Development Group Corporate Seminar,
  • "Novel ELP-intein based purifications (affinity methods)." 2009, Presented at 15th International Conference on Biopartitioning and Purification,
  • "Single-Step Protein Purification Using Controllable Self-Cleaving Affinity Tags." 2001, Presented at Protein Expression Meeting,
  • "Rapid Single-Step Protein Purification Using Self-Cleaving Binding Domains." 2001, Presented at Chemical Engr Conference for Collaborative Research in the Eastern Mediterranean,
  • "Self-cleaving Non-chromatographic Purification Tags: Current Results and Future Prospects." 2010, Presented at BioProcess International,
  • "Recombinant protein purification using self-cleaving tags: Novel twists on conventional and non-chromatographic methods." 2010, Presented at Recovery Of Biological Products XIV,
  • "A Self-Cleaving Tag Method for the Purification of Complex Glycoproteins." 2015, Presented at BioInnovation Leaders Summit,
  • "Putting Biology into Bioseparations: Genetically Engineering a Self-Purifying Protein Expression System." 2002, Presented at UEF Conference on Process Innovation and Process Intensification,
  • "Self-Associating Self-Cleaving Tags for Protein Purification." 2007, Presented at BioProcess Internationalâ„¢ Conference & Exhibition,
  • "Featured Presentation: Tag Technologies: Opportunities and Challenges in the Biopharmaceutical Industry." 2010, Presented at The Bioprocessing Summit: Affinity Tag Protein Purification,
  • "Generation, Mechanisms and Application of Chimeric Allosteric Hormone Sensors." 2007, Presented at International Conference on Biomolecular Engineering (SBE),
  • "A bacterial sensor for peroxisome proliferator-activated receptors gamma ligands." 2009, Presented at The 238th ACS National Meeting,
  • "FAST and CHEAP Protein Purification: Two Novel Technologies for Recombinant Protein Purification Using Simple Centrifugation." 2005, Presented at Mid Atlantic Bioengineering Consortium Conference,
  • "Engineering allosteric inteins for use as biosensors or in bioseparations." 2007, Presented at The 234th ACS National Meeting,
  • "Elastin-like polypeptide-intein mediated protein purification." 2007, Presented at The 234th ACS National Meeting,
  • "Optimization of elastin-like polypeptide sequences for better genetic stability." 2009, Presented at The 238th ACS National Meeting,
  • "Large scale production and purification of elastin-like polypeptide tagged organophophate hydrolase." 2010, Presented at The 239th ACS National Meeting,

Papers in Proceedings

2014

  • Baretto,Jeevan; Wood,David "Detection of novel therapeutic compounds using a simple protein-based bacterial biosensor." in 247th National Spring Meeting of the American-Chemical-Society (ACS). (3 2014).
  • Miskioglu, EE, Wood, DW "That’s Not My Style: Understanding the Correlation of Learning Style Preferences, Self-Efficacy, and Student Performance in an Introductory Chemical Engineering Course." in IEEE Frontiers in Education 2014. (10 2014).

2013

  • Baretto,Jeevan; Li,Jingjing; Wood,David "Neurexin-based biosensor to detect potential ligands." in Symp on Physicochemical Characterization of Organic Matter: Past, Present, Future, and Role of Environment / 245th Natl Spring Meeting of the Amer-Chem-Soc (ACS). (4 2013).

2008

  • Mee,Courtney; Banki,Mahmoud,Reza; Wood,David,W "Towards the elimination of chromatography in protein purification: Expressing proteins engineered to purify themselves." in Process Intensification and Innovation Process Conference. (1 2008).

2005

  • Banki,M,R; Wood,D,W "Novel technology for recombinant protein purification: One-step affinity purification using simple centrifugation.." in 229th National Meeting of the American-Chemical-Society (ACS). (3 2005).
  • Banki,M,R; Wood,D,W "Reducing the cost of affinity purification: In vivo affinity matrix production and application of an engineered self-cleaving intein.." in 229th National Meeting of the American-Chemical-Society (ACS). (3 2005).
  • Skretas,G; Wood,D,W "Post-translational regulation of protein activity with small-molecule-controlled peptide splicing.." in 229th National Meeting of the American-Chemical-Society (ACS). (3 2005).
  • Skretas,G; Wood,D,W "Engineering hormone-sensitive bacteria." in 229th National Meeting of the American-Chemical-Society (ACS). (3 2005).

2003

  • Wood,D,W "Simplified protein purification using engineered self-cleaving affinity tags." in Process Innovation and Process Intensification Conference. (2 2003).