Jeff Chalmers Laboratory for Cell Analysis and Separation

Mankind is currently experiencing a revolution in our understanding of biological processes, from those occurring in “simple” bacteria to those occurring in human beings. Like other scientific revolutions of the past, rapid scientific advances lead to rapid development of engineering disciplines to assist in the understanding and application of this new knowledge. We are seeing this take place today in the development of the discipline of “bioengineering.”

My research is in the area of bioengineering. I have current interest/ projects in the broad areas of cell analysis/characterization/detection, cell separation, and cell culture, ranging from the study of human stem and cancer cells to industrially relevant cells. I also have interest in the emerging area of functional genomics, especially as it is related to cell culture, cell separation, and cancer cells.

VIDEO AT LEFT: confocal, microscopic images are shown of an actual circulating tumor cell isolated from the blood of a head and neck cancer patient. The nucleus is blue, and the green color is staining internal proteins consistent with a cancer cell.

Jeffrey Chalmers

Ph.D., Cornell University, 1998

Professor Chalmers' research foci include iron metabolism, Intrinsic magnetization susceptibility of cells, cell separation, sickle cell detection/ treatment, anemia, immunomagnetic cell separation, cancer detection, and bioengineering.

SELECTED ACHIEVEMENTS

exploiting intrinsic magnetic susceptibility for cell characterization and separation
characterization of the effect of hydrodynamic forces on cells

Major innovations in bioprocessing technologies for the 21st century, including:

Further using an intrinsic properties of many cells, magnetic susceptibility, as both a signal to characterize the cells, and in a number of applications, separate the cells. A specific example, beyond focusing on red blood cells, is ferroptosis, an iron related cell death pathway that is gaining interest in cancer research.
Progressively working towards the quantification of hydrodynamics forces in bioprocessing and its relationship to the hydrodynamic sensitivities frequently used in mammalian cell lines
Designing a microfluidic apparatus that approximates the hydrodynamic conditions in bioprocess equipment through which shear sensitivity of different cell lines at different culture conditions can be quantified, enabling him to be among the first to quantitatively show that mammalians of these cell lines are very robust mechanically in the context of bioprocessing.

In the coming decades, cell separations will become increasingly important for the next wave of biologics, both for examining diseased cells as well as treating disease with cell therapies.

Chalmers has 13 patents.

KEY DISTINCTIONS

INTERNATIONAL

Engineering Conference International:

Cell Culture Engineering Award (bi-annual, 2014)
Cell Culture Engineering Award lecture, 2016

NATIONAL AWARDS

American Association for the Advancement of Science:

Fellow, 2005

American Institute of Chemical Engineers:

15C plenary lecture, 2005

American Institute for Medical and Biological Engineering:

Fellow, 2001

National Science Foundation:

Young Investigator Award, 1992-97

REGIONAL

Ohio Agricultural Research and Development Center and The Ohio State University

Distinguished Multidisciplinary Team Research Award, 1996

The Ohio State University College of Engineering:

Lumley Research Award, 2011, 2006, 2001, 1997, 1993
Harrison Award, 2003
Research Accomplishment Award, 1998

ADDITIONAL HONORS:

Sigma Xi, Phi Beta Kappa, Tau Beta Pi:

Member

Chronological List:

Cell Culture Engineering Award lecture, May 2016
Cell Culture Engineering Award, Engineering Conference International, 2014 (global, bi-annual award)
College of Engineering Lumley Research Award: 2011, 2006, 2001, 1997, 1993
Fellow, American Association for the Advancement of Science, 2005
AIChE 15C plenary lecture, 2005
College of Engineering Harrison Award, ($14,000), 2003
Fellow, American Institute for Medical and Biological Engineering (AIMBE), 2001
College of Engineering Annual Research Accomplishment Award, 1998
Distinguished Multidisciplinary Team Research Award, 1996, Ohio Agricultural Research and Development Center and The Ohio State University
NSF National Young Investigator Award, 1992-1997
Member, Tau Beta Pi, Phi Beta Kappa, Sigma Xi

Chalmers, Jeffrey

Endowed Chair, Chemical and Biomolecular Engineering

Directory Categories

Faculty / Research Area

-Bioengineering, Biotechnology

(614) 292-2727

chalmers.1@osu.edu

chalmers magnetic separation of blood cells — MAGNETIC SEPARATION OF BLOOD CELLS
a) Diagram of the magnetic cell separation system. Consistent with the fluorescent cell tracker dyes used in the separation studies, blue and red circles representing the locations of the cancer stem cells and non-stem tumor cells are included.
(b) Microscopic images of the stained cells collected in flow through exit stream, and the cells deposited on the wall and subsequently washed out.

I am a member of the Translational Therapeutics Program at the OSUCCC – James, where I bring a special perspective from the Chemical and Biomolecular Engineering Department in the College of Engineering. My research aids in the development of fundamental understanding and applications of the interaction of hydrodynamic and magnetic forces and cells. It ranges from the establishment of mathematical relationships detailing physical phenomena to the development of simple, robust instruments to use in a clinical setting.

Results of one of my recent studies indicate that miR-21 and miR-29a are necessary for pro-tumor functions of myeloid cells, and that the CSF1-ETS2 pathway upstream of the miRs acts as a therapeutic target to inhibit M2 remodeling of macrophages during malignancy. I have co-authored numerous articles in such well-respected publications as Breast Cancer Research, Methods, Journal of Magnetism and Magnetic Materials, and Frontiers in Oncology.

Within the area of bioengineering. I have current interest/ projects in the broad areas of cell culture, ranging from the study of human stem and cancer cells to industrially relevant cells. I also have interest in the emerging area of functional genomics, especially as it is related to cell culture, cell separation, and cancer cells.

One specific project involves the ability to identify and separate cells based on specific immunological markers. In collaboration with a colleague at the Cleveland Clinic Foundation, we have developed three new, patented immunomagnetic instruments. One of these instruments is an analytical device, while the other two are separation instruments. All three of these instruments have a large number of applications as indicated by the research support that includes the National Cancer Institute, the National Science Foundation, the Whitaker Foundation, and a private corporation. Some of these applications include: human stem cell separation for bone marrow transplants, isolation/separation of rare cancer cells circulating in human blood, and identification and separation of genetically engineered cells with specific phenotypes. Besides studying the engineering principles of this separation approach, exciting fundamental and applied biological questions are being addressed with respect to these isolated cells using the latest molecular techniques.

Another research area involves the development of better fundamental understanding and scale-up criteria for bioprocesses using animal cells. While large-scale animal cell culture has become quite common, a number of important questions/issues remain unresolved. For example, what is the optimum hydrodynamic environment for a suspended or anchorage- dependent cell? What is the mechanism of action, from a molecular point of view, of surface active agents in cell culture medium? Finally, my research group is exploring collaborations with systems research faculty to develop approaches to understand the great wealth of genetic information which is rapidly becoming available. This research will have specific applications with respect to the cancer cell separation projects as well as more general interest.

Restricted Funding (Diversity Supplement-C. Gilbert): Fractionation of agaed RBCs based on hemoglobin content. 7/12019-12/31/2019.
A scalable platform to selectively purify engineered extracellular vesicles via self-cleaving tags. National Inst. Gen Medical Scis. R21GM126543, 7/1/2019-6/31/2021. Role: co-PI with David Wood.
Fractionation of Aged RBCs Based on Hemoglobin Content. NHLBI, 1R01 HL131720-01A1, 1/1/17-12/31/21 (Role: co-PI with Palmer).
Multi-color immunohistochemistry staining of Genentech cells. 10/31/2015-4/31/2016.
Microcarrier cell shear collaboration, Millipore Merck, RF 60046284. 1/1/2015-10/31/2015.
Role of tumor-associated endothelial cells in chaperoning tumor cells. NCI –R21CA17864. 7/2-14-6/30/16. (Role: Investigator)
Targeted delivery of miroRNA-loaded microvesicles for cancer therapy. NIH UH2TR000914-01. 1/1/2014-12/31/2019. Role: Co-Investigator
Micellular Electrospray Synthesis of Magnetic Quantum Dots. NSF# 1206745. (PI: Jessica Winter, coPIs: Barbara Wyslouzil (OSU), Jeffrey Chalmers (OSU), Gang Ruan (OSU)), $359,779. 7/10/2012-6/30/2015.
Magnetic technologies for microalagal biofuel production. GRT00025262 (PI of sub-contract: Chalmers). NSF Phase II SBIR awarded to Phycal, Inc. 4/31/12- 3/31/14
CTC blood testing and Analysis. Navel Health Research Center (W911QY-10-C-0235). Chalmers, P.I. $180,000, 1/1/2011-9/29/2013.
Characterization of Millipore disposable bioreactor, Millipore Corporation. Chalmers, P.I. $50,000. 9/1/2010-1/13/2011.
Large-scale human placenta progenitor cell-derived erythrocyte production – continuous red blood cell production. Celgene Corp./DARPA, Phase II, Chalmers P.I., $1,000,000. 1/1/10-07/31/12.
(ARRA) CellTrap: A novel solid phase platform for analysis of stem/progenitor cells. Moldovan P.I. (RC2 AG036559) National Institute on Aging $676,675, 9/1/2009-8/31/2010.
Fluorescent-magnetic nanomaniputators for cytoskeletal mechanical investigations. Winter P.I, Chalmers Co-P.I. NSF GRT00013770, $313,433, 6/1/09-5/31/2012.
Characterization of Millipore disposable bioreactor, Millipore Corporation. Chalmers, P.I. $50,000. 1/1/2009-1/31/2010.
Large-scale human placenta progenitor cell-derived erythrocyte production – continuous red blood cell production. Celgene Corp./DARPA, Chalmers P.I., $313,739, 9/7/08- 9/7/09.
CCLI: Educational materials to enhance chemical engineering curricula with applications in biological engineering. NSF Division Undergraduate Education (DUE-0816823) Komives, P.I., Chalmers, P.I. of sub-contract. $33,810.00, 7/1/2008-07/31/12.
Commercialization of Magnetic Nanobeads for Cancer Cell Enrichment. Columbus Nanoworks Inc subcontract from NIH, Chalmers P.I. of Subcontract. $7,500. 7/1/08-9/30/09.
Magnetic Cell Sorting and Analysis. NIH, (2R01 CA062349-15), Zborowski, P.I., Chalmers subcontract, 2/1/11-1/30/16. $716,118.
Intracellular nanoprobes for physical manipulation of cells. NSF Div Chem, Bioeng, Environ, & Transp S (35000309): CBET-0707969, J. Winter, P.I., Chalmers, Co-P.I. 06/15/2007 - 05/31/2008 $129,737.
Advanced Biomedical Devices for Disease Diagnosis and Therapy. State of Ohio Third Frontier Commission, $3,500,000, 7/1/06-6/31/09.
Magnetic nanobeads for cancer cell separation, Awarded to Columbus Nanoworks, NCI SBIR, 1R43CA116048-01, 7/1/05-12/31/05. $37,000 to OSU
Magnetic sorter flow channels for pancreatic islet isolation, NIDDK-SBIR Phase I, R44 HL74606-02, 7/1/05-12/31/05. $16,000 to OSU,
Magnetic Sorter Channels for Stem Cells, NHLBI-SBIR Phase II (1 R43 DK072647-01), (SHOT/Paul Todd, P.I.; Chalmers P.I. of sub-contract), 7/1/05-6/30/07. $40,000 to OSU
Algal Shear Sensitivity, Martek Biosciences Corp. 4/1/05-4/1/07. $50,000.
NSEC Proposal for a Center for Affordable Nanoengineering of Polymer Biomedical Devices, NSF EEC-0425626, 9/04-8/09; $12,900,000; (P.I. Jim Lee; Chalmers, Co-P.I.).
An Integrated Magnetic Cell Identification System, DOD, subcontract from Cleveland Clinic Foundation, $308,000, 5/04-4/07.
Magnetic Separation of Liberated Islets During Isolation, 1R01DK068757-01; 8/04-7/06. $209,838.
Cell Selection by Magnetic Flow Sorting, NIH-NCI, RO1 CA62349, 2/04-2/08, (P.I. Zborowski, Chalmers P.I. of sub-contract to OSU), $300,000.
QMS Technology to Deplete T Cell Alloreactivity, NIH-NIAID, 1 R01 AI056318-01, 3/04-2/09, (P.I. Farag, S., Chalmers, Co-P.I.), $3,200,000.
Magnetic Sorter Channels for Stem Cells, NHLBI-SBIR (1 R43 HL074606-01), 7/1/03-12/31/03, (SHOT/Paul Todd, P.I.; Chalmers P.I. of sub-contract), $6,000.
Magnetic Sorter Channels for Rare Cancer Cells, NSF SBIR (NSF 02-056; Proposal # 79440), 7/1/03-2/04, (SHOT/Paul Todd, P.I; Chalmers P.I. of sub-contract) $25,000.
High Performance Magnetic Cell Sorting. National Cancer Institute, 1 R01 CA97391-01A1 (PI: Chalmers) 5/03 - 4/07. $2,098,000.
The development of theoretical and experimental characterizations of the magnetophoretic mobility of immunomatically labeled cells. National Science Foundation, (BES-0124897) 7/1/02 - 6/31/05, $481,121 Chalmers, (P.I.); Zborowski, M., P.I. of sub-contract to CCF.
Development of Quadrupole Magnetic Field-Flow Fractionation: Application to Characterization of Magnetic Colloids and Microparticles, National Science Foundation, (CTS-0125657), 3/1/02-2/28/05, $289,207, Williams, P.S. (P.I.), Zborowski, M., Chalmers, J. Co-P.I. ($97,272 to O.S.U.)
OSU Comprehensive Cancer Center Support Grant, National Cancer Institute, 7/01/77-11/30/10, (5 P30 CA16058-26) Caliguiri, M. (PI), Chalmers, Investigator and Director of Analytical Cytometry Core, $151,000/yr.
Cell Selection by Magnetic Flow Sorting, National Cancer Institute, (RO1 CA62349) 1/00 - 1/31/04, $1,440,750; Zborowski, M. (P.I).; Chalmers P.I. of subcontract to OSU.
Nanostructured Biocomposities for Use as Novel Films and Coatings. National Science Foundation, 2/00-1/02, $100,000; Rathman, J (P.I.); Chalmers, J.
Rapid Rare Cancer Cell Isolation For Molecular Diagnosis. National Cancer Institute, (R33CA81662) 4/99 - 3/02, $1,050,000; Chalmers, J. (P.I.); Zborowski P.I. of sub-contract to CCF.
Dynamic Mixing in Stirred Vessels: An Application of Particle Tracking Velocimetry and Flow Visualization in a Convected Frame of Reference. National Science Foundation GOALI program, 9/99- 12/02, $90,000 ($70,000 from NSF) Brodkey, R. (P.I.); Chalmers J.J.; Guezennec, Y.
Determination of Cellular Properties based on the distribution of cell velocities induced by external fields, National Science Foundation, (BES-9731059) 9/98- 7/01 (extended to 02), $460,180, Chalmers (P.I.), Zborowski P.I. of subcontract to CCF.
Magnetic Flow Sorting for Clinical Stem Cell Isolation. NOVAMEDICS (A Venture Capital Firm), 5/98-5/99, $375,000. Zborowski, M., Chalmers P.I. of sub-contract of to OSU.
Improved Surfactants for Cell Culture. NIH SBIR Phase I with Anatrace Inc., 1/1/98-12/31/98, $100,000, ($37,500 to P.I.)
Characterization of bench and pilot scale bioreactors with respect to their hydrodynamic environment. Genentech, 10/1/97-8/31/98, $22,360.
Development of Analog Cell Separation Based on Surface Marker Density, Whitaker Foundation, 4/1/97-4/1/98. $66,000.
Cell Selection by Magnetic Flow Sorting, National Cancer Institute, Division of the National Institute of Health, $514,902; Zborowski, M. (P.I.), Chalmers, J. 3/97-1/00.
Ohio Bioprocessing Research Consortium, Ohio Board of Regents, $1,450,000, Yang, S.T. (P.I.); Chalmers, J., and contributions from Battelle and U. of Akron. 1996.
Engineering Research Equipment: A Rheological Measurement System for Polymers and Composite Processing, and Rheology of Colloids, Emulsions, Polymers Solutions, and Biomaterials, Lee, J., Zakin, J., Koelling, K., Rathman, J., Chalmers, J. National Science Foundation, $360,000, 8/94.
Dynamics of Biological Energy and Nitrogen Availability During Composting/Utilization, Targeted Interdisciplinary Seed Grant Award, The Ohio State University Office of Research, $84,975, 1994-96, Co-P.I.'s H.A. Hoitink, W.A. Dick, H. M. Keener
Renovation of Laboratories for Biochemical and Bioprocess Engineering Research, National Science Foundation, $234,199 (total award with match $468,398), 1994, Yang, S.T., Zakin, J., Chalmers, J.
Continuous Magnetic Cell Sorting, National Cancer Institute, (RO1 CA62349), Zborowski, M. (P.I.), Chalmers, J., $550,000, 1994-97.
Development of a Continuous, Magnetic Cell Separator, Whitaker Foundation, $180,000, 12/93-12/96, Chalmers, J., (P.I.), Zborowski, M.
Applications and Development of Biochemical Engineering Principles to the Production of Soil Amendments, The Consortium for Plant Biotechnology Research, Inc., 7/93-6/95, $95,128
National Young Investigator Award, National Science Foundation, (BCS9258004) 7/92-7/98, $237,500.
Cargill Inc., 5/93-6/94, $11,000
AMGEN Inc., 1/93, $10,000; 12/92, $4,500 in equipment
Eli Lilly, 5/93, $3,500; 12/91, $3000
Chemineer, 5/93, $2,500
Determination of the Mechanisms of Cell Adhesion to Damage from Air-Liquid Interfaces, Principal Investigator with Dr. W.F. Hink (Entomology), National Science Foundation, 7/91- 7/93, $137,300.
DuPont Young Faculty Development Award, 1989-1991, $75,000, joint with Dr. S.T. Yang.
Studies on the Effect of Hydrodynamic Shear Stress on Suspended Insect Cells, 3/89 to 9/90, Seed Grant, Office of Research and Graduate Studies, Ohio State University, $16,000.

CBE Department News

Books

Zborowski, M. and Chalmers, J. J. Magnetic Cell Separation, Vol. 32 of Series: Laboratory Techniques in Biochemistry and Molecular Biology, Elsevier, Amsterdam, 2007.

Chapters

Frank, G, Chalmers, J.J., Harrison, R.G., Todd, P.W., Rudge, S., Petrides, D.P., Pepper, C., Downey, B., Breit, J., Betenbaugh, M.J., Calzadilla, N. “Bioreactions and Bioprocessing.” Section 20, 9th Edition, Perry’s Chemical Engineers’ Handbook, In Press.
Miller, B.L., Balasubramanian, P., Summers, T.A. Chalmers, J.J., Lustberg, M.B. “Spectral Deconvolution analysis of multicolor, fluorescently stained, negatively enriched, peripheral blood from cancer patients containing circulating tumor cells.” Submitted to Methods
Frank, G, Chalmers, J.J., Harrison, R.G., Todd, P.W., Rudge, S., Petrides, D.P., Pepper, C., Downey, B., Breit, J., Betenbaugh, M.J., Calzadilla, N. “Bioreactions and Bioprocessing.” Section 20, 9th Edition, Perry’s Chemical Engineers’ Handbook, In Press (2016).
Zborowski, M., Chalmers, J.J., “Magnetic Cell Manipulation and Sorting,” Microtechnology for Cell Manipulation and Sorting. W.Lee, P. Tseng, D. DiCarlo, eds., Springer (2017).
Jeffrey J. Chalmers, Maryam B. Lustberg, Clayton Deighan, Kyoung-Joo Jenny Park, Yongqi Wu, Peter Amaya, Depletion of normal cells for CTC enrichment, Circulating Tumor Cells: Isolation and Analysis. John Wiley & Sons, 2015.
Zborowski, M., Chalmers, J.J., Magnetophoresis: Fundamentals and Applications. Wiley Encyclopedia of Electrical and Electronics Engineering. John Wiley & Sons, 2015.
Chalmers, J. J, Ma, N., Hydrodynamic Damage to Animal Cells, Cell Engineering, Animal Cell Culture, Vol 9:169-183. 2014.
Lustberg, M., Jatana, K., Zborowski, M., Chalmers, J.J. Emerging Technologies for CTC detection Based on Depletion of Normal Cells. In: Minimal Residual Disease and Circulating Tumor Cells in Breast Cancer, Ed: K. Pantel, C. Sotiriou, M. Ignatiadis, Springer-Vertag GmbH, Berlin. 2012.
Godoy-Silva, R., Berdogo, C., Chalmers, J.J. Aeration, Mixing, and Hydrodynamics in Bioreactors. In: Wiley Encyclopedia of Industrial Biotechnology, 2010.
Xiong, Y. Shao, M., Zborowski, M., Chalmers, J.J. Magnetic cell separation to enrich for rare cells. In: Methods in Bioengineering (MIB), Vol. Editor K. Rege, Series editors: Yarmush, M and Langer, R.S. Artech House. 2009.
Ma, Ningning, Mollet, M., Chalmers, J.J. Aeration, Mixing, and Hydrodynamics in Bioreactors. In: Cell Culture Technology for Pharmaceutical and Cellular Therapies. CRC Press, Taylor and Francis Group, Boca Raton, FL. 2006, Pp. 275-304.
Lara, O. Chalmers, J.J. Cell enrichment and immunochemical staining. In: Immunochemical Protocols, Methods in Mol. Biol. Humana Press; Vol 295:301-10. 2005. PMID: 15596905.
Zborowski, M., Chalmers, J.J. Magnetic Cell Sorting, In: Immunochemical Protocols, Methods in Mol. Biol. Humana Press; Vol 295:301-10. 2005.
Zborowski, M., Williams, S, Chalmers, J.J., Magnetic FFF, Encyclopedia of Chromatography, Marcel Dekker, Inc. New York, 2001. Pp.503-507.
Chalmers, J.J. Animal Cell Culture, Effects of Agitation and Aeration on Cell Adaptation, In: Encyclopedia of Cell Technology, R. Spier, J.B. Griffiths, A. H. Scragg, eds. 2000, Wiley, New York. Pp. 41-51.
Zborowski, M., Moore, L.R., Sun, L., Chalmers, J.J., Continuous-flow Magnetic Cell Sorting Using Soluble Immunomagnetic Labels, In: Scientific and Clinical Applications of Magnetic Carriers, W. Schuett, U. Hafeli, J. Teller, M.Zborowski eds. (1997), Plenum Press.
Chattopadyay, D., M. Garcia-Briones, R. Venkat, Chalmers, J.J., Hydrodynamic Properties in Bioreactors, In: Mammalian Cell Biotechnology in Protein Production, Hansjorg Hauser, Roland Wagner, (1997) ed., Walter de Gruyter, Publishers, Berlin.
Chalmers, J.J., Shear Sensitivity of Insect Cells, In: Insect Cell Cultures: Fundamental and Applied Aspects, Just Vlak, et al. (eds). (1996) Kluwer Academic Publishers.
Chalmers, J. J., The Effect of Hydrodynamic Forces on Insect Cells, In: Insect Cell Culture: Production of Improved Biopesticides and Proteins From Recombinant DNA, Michael Shuler (1995), ed. Hanser Publishers, New York.
Shuler, M.L., Kim, B.G., Chalmers, J.J., Kim, E., Wilson, D.B. On Bioprocess Considerations and the Use of Genetically Modified Escherichia coli- systems for excretion of plasmic-encoded proteins, In: Bioprocess Engineering: 1st Generation, Trarum Ghose (1989), ed., Ellis Horwood Limited.

Visit Google Scholar for more information about publications from the Chalmers Laboratory.

Publications Cited 100 or More Times

McCloskey, K., Chalmers, J.J., Zborowski, M., Magnetic Cell Separation: Characterization of Magnetophoretic Mobility, Analytical Chemistry 75(4):6868-6874. 2003. PMID:14670047.
Zborowski, M., Ostera, G.R., Moore, L.R., Milliron, S., Chalmers, J.J., Schechter, A.N. Red Blood Cell Magnetophoresis, Biophysics Journal, 84:2638-2645. 2003.
Zborowski, M., Sun. L., Moore, L.R., Williams, P.S., Chalmers, J.J. Continuous cell separation using novel magnetic quadrupole flow sorter, J. of Magnetism and Magnetic Materials. 194:224-230, 1999.
Lara, O., Tong, X., Zborowski, M., Chalmers, J.J. Enrichment of Rare Cancer Cells through Depletion of Normal Cells Using Density and Flow-Through, Immunomagnetic Cell Separation. Experimental Hematology Oct;32(10):891-904. 2004. PMID: 15504544
Yang, L., Lang, J.C., Balasubramanian, P., Jantana, K.R., Schuller, D., Agrawal, A., Zborowski, M., Chalmers, J.J. Optimization of an Enrichment process for Circulating tumor cells from the blood of Head and Neck Cancer patients through depletion of normal cells. Biotechnol. Bioeng. 102 (2):521-534. 2009. PMID: 18726961
Agarwal, M., Koelling, K., Chalmers, J.J., Characterization of the Degradation of Poly-Lactic Acid in a Well Controlled Composting System, Biotechnology Progress, Vol. 14:517-526, 1998.
Chalmers, J., Zborowski, M., Sun, L., Moore, L., Flow Through, Immunomagnetic Cell Separation, Biotechnology Progress, Vol. 14:141-148, 1998.
Zborowski, M, Chalmers, J.J. Rare Cell Separation and Analysis by Magnetic Sorting. Analytical Chemistry 83(21):8050-8056. 2011. PMID:21812408; PMCID:3205221; NIHMS319811
Ma, N., Koelling, K., Chalmers, J.J. The Fabrication and Use of A Transient Contraction Flow Device to Quantify the Sensitivity of Mammalian and Insect Cells to Hydrodynamic Forces, Biotechnology and Bioengineering 80:428-437. 2002; PMID: 15296446.
Zhu, X., Badawi, M., Pomeroy, S. M.,Sutaria, D.S., Xie,, Z., Baek, A., Jiang, J., Elgamal, O J, Mo, X., Perle, K.L., Chalmers, J.J, Schmittgen, T.D., Phelps, M. Comprehensive toxicity and immunogenicity studies reveal minimal effects in mice following sustained dosing of extracellular vesicles derived from HEK293T cells, Journal of Extracellular Vesicles 6(1), 1324730. 2017.
Chalmers, J.J., Kim, E., Telford, J.N., Shuler, M.L., Wilson, D.B., Effects of Temperature on the Stability of Escherichia coli Overproducing β-lactamase or Human Epidermal Growth Factor, Applied and Environmental Microbiology, 56:104-111, 1990.
Zborowski, M., Fuh, C.B., Green, R., Sun, L., Chalmers, J.J., Analytical Magnetopheresis of Ferritin-labeled Lymphocytes, Analytical Chemistry, 67(20):3702-3712, 1995
Chalmers, J.J., Bavarian, F., Microscopic Visualization of Insect Cell-Bubble Interactions II: The Bubble Film and Bubble Rupture, Biotechnology Progress, 7:151-158, 1991.
Hu, W. Berdugo, C., Chalmers, J.J. The potential of hydrodynamic damage to animal cells of industrial relevance: current understanding. Cytotechnology 63:445-460. 2011. PMID: 21785843.
Zborowski, M. Chalmers, J.J. Magnetic cell separation, 2011. Elsevier.
Sun, L., Zborowski, M., Moore, L., Chalmers, J.J., Continuous, Flow-Through Immunomagnetic Cell Separation in a Quadrupole Field. Cytometry. 33:469-475. 1998.
Vieira, G., Heninghan, T., Chen, A., Hauser, A.J., Yang, Y., Chalmers, J.J., Sooryakumar, R. Magnetic wire traps and programmable manipulation of biological cells. Physical Review Letters, 103:128101, 2009.
Jing, Ying, Moore, LR, Schneider, T., Williams, PS, Chalmers, JJ., Farag, SS, Bolwell, B., Zborowski, M. Blood progenitor cell separation from clinical leukapheresis product by magnetic nanoparticle binding and magnetophoresis Biotechnol Bioeng. 96(6):1139-1154, 2007. PMID: 17009321
Jatana, KR, Balasubramian, P., Lang, JC, White, E., Agrwal, A., Ozer, E., Schuller, DE, Teknos, TN, Chalmers, JJ. Significance of circulating tumor cells in patients with squamous cell carcinoma of the head and neck. Archives of Otolaryngology--Head and Neck Surgery, 136(12):1274-1279. 2010. PMID: 21173379
Bavarian, F., Fan, L.S., Chalmers, J.J., Microscopic Visualization of Insect Cell-Bubble Interactions I: Rising Bubbles, Air-Medium, and the Foam Layer, Biotechnology Progress, 7:140-150, 1991.
Balasubramanian, P., Lang, J.C., Jatana, K., Miller, B., Schuller, D., Agrawal, A., Lustberg, M., Zborowski, M., Chalmers, J.J. Multiparameter analysis, including EMT markers, on negatively enriched blood samples from patients with squamous cell carcinoma of the head and neck. PLoSONE. 7:1-11, e42048, 2012; PMID:22844540; PMCID: PMC3406036.
Gregoriades, N., Clay, J., Ma, Ningning, Koelling, K., Chalmers, J. Cell Damage of Microcarrier Cultures as a function of Local Energy Dissipation created by a Rapid Extensional Flow, Biotechnology and Bioengineering 69:171-182, 2000. PMID: 10861396
Chalmers, J.J., Zhao, Y., Nakamura, M., Melnik, K. Lasky, L., Moore, L., Zborowski, M., An Instrument to determine the magnetophoretic mobility of paramagnetic particles and labeled, biological cells. J. of Magnetism and Magnetic Materials. 194:231-241, 1999.
Moore, L.R., Fujioka, H., Williams, P.S., Chalmers, J.J., Grimberg, B., Zimmerman, P., Zborowski, M., Hemoglobin degradation in malaria-infected erythrocytes determined from live cell magnetophoresis; FASEB J. 2006 Apr;20(6):747-9.
Mollet, M., Ma, N., Zhao, Y., Brodkey, R., Taticek, R., Chalmers, J. Bioprocess Equipment: Characterization of Energy Dissipation Rate and its potential to Damage Cells. Biotechnology Progress 20:1437-1448. 2004; PMID: 15458328

ADDITIONAL REFEREED PUBLICATIONS

Kim^,J., Gómez-Pastora^,J., Weigand^,M., Walters^,N.A., Reátegui^,E., Palmer^,A.F., Yazer^,M., Zborowski^,M., Chalmers, J.J. Potential of Cell Tracking Velocimetry as an Economical and Portable Hematology Analyzer, Submitted to Experimental Hematology.
Gómez-Pastora, J, Kim, J., Multane, V., Weigand, M., Walters, N.A., Reategui, E., Palmer, A.F., Yazer, M., Zborowski, Z., Chalmers, J.J. Intrinsically magnetic monocytes subtypes: Non-classical and intermediate monocytes have the strongest magnetic behavior in fresh human blood. Experimental Hematology Vol. 99, p21–3, 2021
Weigand, M.R.H., Gómez-Pastora, J., Kim, J., Kurek, M., Hickey, R., Irwin, D.C., Buehler, P.W., Zborowski, M., Palmer. A., Chalmers, J.J. Magnetophoretic and Spectral Characterization of Oxyhemoglobin to Deoxyhemoglobin: Chemical vs Enzymatic Process, Accepted in PlosOne.
González Fernández, Cristina, Gómez Pastora, Jenifer, Basauri, Arantza, Fallanza, Marcos, Bringas, Eugenio, Chalmers, Jeffrey J., Ortiz, Inmaculada, Continuous-flow separation of magnetic particles from biofluids: how does the microdevice geometry determine the separation performance?, Sensors, 20:3030. 2020.
Roodan, V.A., Gómez-Pastora, J, Karampelas, I.H., Gonzalz-Fernandez, C., Bringas, E., Ortiz, I., Chalmers, Jeffrey J. Fulrani,E.P., Swihard M.T. Formation and manipulation of ferrofluid droplets with magnetic fields in a microdevice: a numerical parametric study, Soft Matter, Oct 28; 16(41):9506-9518. 2020.
Gómez-Pastora, J, Mitchell Weigand; James Kim; Xian Wu; Jacob Strayer; Andre F Palmer; Maciej Zborowski; Mark Yazer; Jeffrey J Chalmers, Hyperferritinemia in critically ill COVID-19 patients - Is ferritin the product of inflammation or a pathogenic mediator? Clinica Chimica Acta, Oct. 509:249-251. 2020
Robert Wesolowski, Daniel G Stover, Maryam Lustberg, Abigail Shoben, Meng Zhao, Ewa Mrozek, Rachel M Layman, Erin Macrae, Wenrui Duan, Jun Zhang, Nathan Hall, Chadwick L Wright, Katharina Schregel, Susan Gillespie, Michael Berger, Andrea Camp, Jeffrey J Chalmers, Prija Balasubramanian, Brandon L Miller, Peter Amaya, Eleni Andreopoulou, Joseph Sparano, Charles L Shapiro, Miguel Angel Villalona-Calero, Susan Geyer, Alice Chen, Michael R Grever, Michael V Knopp, Bhuvaneswari Ramaswamy Phase I study of veliparib on an intermittent and continuous schedule in combination with carboplatin in metastatic breast cancer: A safety and [18F]-fluorothymidine PET biomarker study, Oncologist. Aug; 25(8): 3-1158-e1169. 2020
Gómez-Pastora, J, Wu, X., Sundar,N., Alawi,J., Nabar,G., Winter, J.O., Zborowski,Z., Chalmers, J.J. Self-Assembly and sedimentation of 5 nm SPIONs using horizontal, high magnetic fields and gradients, Separation and Purification Technology. Vol. 248, 117012, 2020.
Mahajan, Kalpesh D., Ruan, Gang, Vieira, Greg, Porter, Thomas, Chalmers, Jeffrey J., Sooryakumar, Winter, Jessica O. , Biomolecular Detection, Tracking, and Manipulation using a Magnetic-Quantum Dot Platform, Journal of Materials Chemistry B., 8, 3534-3541. 2020,
Kim^,J. Gómez-Pastora^,J., Gilbert^,C., Weigand^,M., Walters^,N.A., Reátegui^,E., Palmer^,A.P., Yazer^,M., Zborowski^,M., Chalmers, J.J. Quantification of the mean and distribution of hemoglobin content in normal human blood using cell tracking velocimetry. Analytical Chemistry. 92(2):1956-1962. 2020.
Kim, J., Gomez-PAstora, J., Weigand, M, Polgoeter. M., Walter, N., Reategui, E., Palmer, A., Zborowski, M., Yazer, M.H., Chalmers, Jeffrey, A subpopulation of monocytes in normal human blood has significant magnetic susceptibility; quantification and potential implications. Cytometry, part A. 95(5):478-487. 2019.
Park, K., Kim, J., Testoff, T., Adams J., Poklara, M., Zborowski, M., Venere, M., Chalmers, J.J., Quantitative Characterization of the Regulation of Iron Metabolism in Glioblastoma Stem-Like Cells using Magnetophoresis. Biotechnology and Bioengineering. 116(7):1644-1655. 2019; PMCID: PMC6693654. 2019.
Kim, James,Weigand, Mitchell, Palmer, Andre F., Zborowski, M., Yazer, M.H., Chalmers, Jeffrey, Single Cell Analysis of Aged RBCs: Quantitative analysis of the aged cells and byproducts, the Analysist. 144(3):935-942. 2019. PMCID: PMC6506859.
Xue, W., Moore, L.R., Nakano, N., Chalmers, J.J., Zborowski, M. Single cell magnetometry by magnetophoresis vs. bulk cell suspension magnetometry by SQUID-MPMS – a comparison. Journal of Magnetism and Magnetic material 474:152-160. 2019.
Aljohani, HM Aljohani, M., Furgason, JM, Amaya, P., Deeb, A., Chalmers, J.J., Bahassi, E.M. The role of Nrf2-Keap1 pathway in the survival of circulating metastatic cancer cell, Cancer Research 78 (13 Supplement), 83-83. 2018.
Lustberg, M.B., Stover, D.G., Chalmers, J.J. Implementing Liquid Biopsies in Clinical Trials: State of Affairs, Opportunities, and Challenges. The Cancer Journal 24(2):61-64. 2018; PMCID: PMC5880324.
Aljohani, HA, Furgason, J.M., Amaya, P. Deeb, A., Chalmers, J.J., Bahassi, E., Genetic Mutations Associated with Lung Cancer Metastasis to the Brain, Mutagenesis. 33 (2), 137-145. 2018.
Moore, L.R., Yazer, M., Zborowski, M., Chalmers, J.J. Continuous, intrinsic magnetic depletion of erythrocytes from whole blood with a quadrupole magnet and annular flow channel; pilot scale study. Biotechnology and Bioengineering. 115 (6):1521-1530. 2018; PMCID: PMC6311700.
Sun, J., Moore, L., Xue, W., Kim, J., Zborowski, M., Chalmers, J.J. Correlation of simulation/finite element analysis to the separation of intrinsically magnetic spores and red blood cells using a microfluidic magnetic deposition system. Biotechnology and Bioengineering. 115 (5): 1288-1300. 2018. PMCID: PMC6338348.
Mahajan, K.D., Cui, Y., Dorcéna, J., Bouxien, N.F., Bachand, G.D., Chalmers, J.J., Winter, J.O, Magnetic Quantum Dots Steer and Detach Microtubules from Kinesin-Coated Surfaces. Biotechnology Journal. 13:1700402, 2018.
Mahajan, K.D., Nabar, G.M., Xue, W., Anghelina, M., Moldovan, N, Chalmers, J., Winter, J. Mechanotransduction effects on endothelial cell proliferation via CD31 and VEGFR2: Implications for Immumomagnetic Separation. Biotechnology Journal. 12(9), 2017.
Similer, J.L. Chalmers, J.J, Hahn, J.3 and Rapiejko, P.J. The Impact of a Flow-through External Circulation Loop on Cell Growth and Metabolism. Accepted in Journal of Biotechnology
Sutaria,D. Jiang,J., Phelps, M. , Elgamal, O., Pomeroy, S. M., Badawi, M. A., Chalmers, J. J. , Clara, A., Zhu, X., Li, C., Low Active Loading of Cargo into Engineered Extracellular Vesicles Results in Inefficient miRNA mimic delivery, Journal of Extracellular Vesicles 6 (1), 1333882. 2017.
Chalmers, J.J., Jin, X., Palmer, A., Yazer, M., Moore, L., Pan, J., Park, J. Zborowski, M., Femtogram resolution of iron content per cell: storage of red blood cells leads to loss of hemoglobin. Analytical Chemistry 89(6):3702-3709. 2017. NIHMSID918420; PMCID:PMC5685515.
Moore, Lee. R., Williams, P.S., Chalmers, J.J., Zborowski, M., Tessellated permanent magnetic circuits for flow-through, open gradient separations of weakly magnetic materials. J. of Magnetism and Magnetic Materials. Vol. 427:325-330. 2017. PMCID:PMC5667671.
Wu, Y., Deighan, C.J., Park, K.J., Amaya, P., Zborowski, M., Lustberg, M., Chalmers, J.J. Multiparmeter Evaluation of the Heterogeneity of Circulating Tumor Cells Using Combined RNA in situ Hybridization and Immunoctyochemical Analysis, Frontiers in Oncology. 6, 2016.
Mahajan, K.D. Ruan, G., Dorcena, C., Vieira, G., Nabar, G., Bouxsein, N., Chalmers, J.J., Bachand, G.D., Sooryakumar, R., Winter, J.O. Steering Microtubule Shuttle Transport with Dynamically Controlled Magnetic Fields. Nanoscale 8:8641-8649. 2016.
KP McMullen, JJ Chalmers, JC Lang, P Kumar, KR Jatana, Circulating tumor cells in head and neck cancer: A review, World Journal of Otorhinolaryngology-Head and Neck Surgery 2 (2), 109-116. 2016.
Jatana, K.R., Balasubramanian, P., McMullen, K.P., Lang, J.C., Teknos, T., Chalmers, J.J. Effect of surgical intervention on circulating tumor cells in patients with squamous cell carcinoma of the head and neck using a negative enrichment technology. Head and Neck. June 5, 2016.
Gallardo-Rodrigguez, J.J., Lopez-Rosles, L., Sanchez-Miron, A., Garcia-Camacho, F., Molina-Grima, E., Chalmers, J.J. New insights into shear-sensitivity in dinoflagellate microalgae. Bioresource Technology. 699-705. 2016.
Chalmers, J.J. Mixing, aeration and cell damage, 30+ years later: what we learned, how did it affect the cell culture industry and what would we like to know more about, Current Opinions in Chemical Engineering, Vol 10:94-102. 2015.
Joao Paulo Oliveira-Costa, Alex Fiorini de Carvalho, Giorgia Gobbi da Silveira1,Peter Amaya3, Yongqi Wu, Kyoung-Joo Jenny Park, Mabel Pinilla Gigliola, Maryam Lustberg, Marcilei Eliza Cavicchioli Buim, Elisa Napolitano Ferreira, Luiz Paulo Kowalski, Jeffrey J. Chalmers, Fernando Augusto Soares, Dirce Maria Carraro, Alfredo Ribeiro-Silva, Gene expression patterns through oral squamous cell carcinoma development: PD-L1 expression in primary tumor and circulating tumor cells. OncoTarget. 28;6(25)20902-20. 2015. PMID26041877.
Barrow, M, Taylor, A ,Nieves, DJ, Bogart, LK,, Mandal, P.,Collins, CM , Moore, LR, Chalmers, JJ, Levy, R Williams, SR, Murray, P, Rosseinsky, MJ, Adams, DJ, Tailoring the surface charge of dextran-based polymer coated SPIONs for modulated stem cell uptake and MRI contrast, Biomaterials Science. 3(4):608-616. 2015. PMID26222421.
Buck, A., Moore, L.R., Lane, C.D., Kumar, A., Stroff, C., White, N., Xue, w., Chalmers, J.J., Zborowski, M., Magnetic separation of algae genetically modified for increased intracellular iron uptake Journal of Magnetism and Magnetic Material. 380:201-204. 2015.
Mathsyaraja, H., Thies, K., Taffany, D.A., Deighan, C., Liu, T., Pecot, T., Yu, L., Fernandez, S.A., Shapiro, C., Otero, J., Timmers, C., Lustberg, M.B., Chalmers, J.J., Leone, G., Ostrowski, M.C. CSF1 Induces Oncogenic microRNAs in Macrophages to Drive Metastatic Tumor Growth. Oncogene 34(28):3651-3661. 2015.
Moore, L., Williams, P. S., Nehl, F., Abe, K., Chalmers, J.J., Zborowski, M. Feasibility study of red blood cell debulking by magnetic field-flow fractionation with step-programmed flow. Analytical and Bioanalytical Chemistry, 406(6):1661-70. 2014.
Lustberg, M., Balasubramanian, P., Miller, B., Garcia-Villa, A., Deighan, C., Wu, Y., Carothers, S., Berger, M., Ramaswamy, B., Macrae, E., Wesolowski, R., Layman, R., Morzek, E., Pan, X., Summers, T., Shapiro, C., Chalmers, J.J. Heterogeneous atypical cell populations are present in blood of metastatic triple negative breast cancer. Breast Cancer Research 16(2):R23. 2014. PMCID: PMC4053256, PMID:24602188
Wu, Y., Clayton Deighan, Brandon Miller, Priya Balasubramanian, Maryam Lustberg, Maciej Zborowski, Jeffrey Chalmers. Isolation and analysis of rare cells in the blood of cancer patients using a negative depletion methodology. Methods. 64(1):169-182. 2013.
Chen, A, Byvank, T., Chang, W-J., Bharde, A., Vieira, G., Miller, B., Chalmers, J.J., Bashir, R., Sooryakumar, R. On-chip Magnetic Separation and Cell Encapsulation in Droplets. Lab Chip 13(6):1172-1181. 2013. NIHMSID: NIHMS619019, PMCID: PMC4176703, PubMed # 23370785
Moore, LR, Nehl, F., Dorn, J., Chalmers, J.J., Zborowski, M. Open Gradient Magnetic Red Blood Cell Sorter evaluation on Model Cell Mixtures, IEEE Transactions on Magnetics 49(1):309-315. 2013.
K.D. Mahajan, G. Vieira, G. Ruan, B.L. Miller, M. Lustberg, J.J. Chalmers, R. Sooryakumar, J.O. Winter (Invited), "MagDot-Nanoconveyer Assay for Detection and Isolation of Molecular Biomarkers," Chemical Engineering Progress, December 2012, 41-51, 2012. PMC 4286893, PMCI: PMC4286893, NIHSID:NIHMS462787
Chalmers, J.J. Magnetic, Batch Separation. Methods Navigator, Cell Biology Protocols Module, Edited by P. Michael Conn. Elsevier 2012.
Miller, B.L., Chalmers, J.J. Cell Enrichment from human blood through red cell lysis. Methods Navigator, Cell Biology Protocols Module, Edited by P. Michael Conn. Elsevier. 2012.
Jie, Xu, Mahajan, K., Xue, W., Winter, J.O., Zborowski, M., Chalmers, J.J. Simultaneous, single particle, magnetization and size measurements of micron sized, magnetic particles, JMMM 324:4189-4199. 2012. PMID: 22962515; PMC3433070.
Garcia-Villa, A., Balasubramanian, P. Miller, B.L., Lustberg, M., Ramaswamy, B., Chalmers, J.J. Assessment of γ-H2AX levels in circulating tumor cells from patients receiving chemotherapy, Front. Oncol. 2:128, 2012; PMID. 23112954; PMC ID PMC3480704.
Jatana, K.R., Lang, J.C., Teknos, T.N., Chalmers, J.J. Circulating tumor cells and squamous cell carcinoma of the head and neck: What do we know and where do we go? In Press in Cancer Treatments- Oncology.
Jin, X., Abbot, S., Zhang, X., Kang, L., Voskinarian-Berse, V., Karmeneva, M.V., Moore, L.R., Chalmers, J.J., Zborowski, M., Erythrocyte enrichment in hematopoietic progenitor cell cultures based on magnetic susceptibility of the hemoglobin. PLoSONE. Vol. 7, Issue 8:1-10, e39491, 2012; PMID: 22952572; PMCID: PMC3428333. 2012
Jin, X., Chalmers, J.J., Zborowski, M. Iron Transport in Cancer Cell Culture Suspensions measured by Cell magnetophoresis. Analytical Chemistry. 84:4520-4526, 2012; PMID 22500468;
Lustberg,, M., Jatana, KR, Zborowski, M., Chalmers, J.J. Emerging Technologies for CTC Detection Based on Depletion of Normal Cells. Recent Results Cancer Res. 195:97-110. 2012.
Jin, X., Yazer, M.H., Chalmers, J.J., Zborowski, M. Quantification of changes in oxygen release from red blood cells as a function of age based on magnetic susceptibility measurements. The Analyst, 136(14):2996-3003. 2011. PMID: 21463135;
Wu, Y. Chalmers JJ, Wyslouzil, B., Casnocha, S., McCormick, E., Ma, N. Growth and productivity of NS0 cells with cholesterol nanoparticle supplementation. Biotechnol Progress. 27(3):796-802. 2011. PMID: 21509955;
Sun, J., Zborowski, M., Chalmers, J. Quantification of both the presence, and oxidation state, of Manganese in Bacillus atrophaeus spores and its imparting of magnetic susceptibility to the spores. Biotechnology and Bioengineering, 108(5):1119-1120. 2011. PMID: 21449026; PMCID: PMC3431158
Jatana, KR, Lang, JC, Chalmers, JJ. Identification of circulating tumor cells: a prognostic maker in squamous cell carcinoma of the head and neck? Future Oncol. 7(4):481-4. 2011. PMID: 21463135; NIHMSID 647365;
Zborowski, M., Moore, L.R., Williams, P.S., Chalmers, J.J. Magnetic Pressure as a Scalar Representation of Field Effects in Magnetic Suspensions. AIP, 2011.
Hu, W., Gladue, R., Hansen, J., Wojnar, C., Chalmers, JJ. Scale-up of algae culture can be limited due to high oxygen demand: It was not shear sensitivity this time! Biotechnol. Progress. 26(1):79-87. 2010. PMID: 19847886
Henighan, T., Chen, A., Vieira, G., Hauser, A.J., Yang, F.Y., Chalmers, J.J., Sooryakumar, R. Transporting Biological Cells via Programmable Mobile Magnetic Traps. Biophys J.. 98(3):412-417. 2010. PMID: 20141754
Schneider, T., Karl, S., Moore, L.R., Chalmers, J.J., Williams, P.S., Zborowski, M. Sequential CD34 cell fractionation by magnetophoresis in a magnetic dipole flow sorter. The Analyst 135(1):62-70. 2010. PMID: 20024182
Chalmers, J,.J., Xiong, Y., Shao, M., Tong, X., Farag, S., Zborowski, M. Quantification of non-Specific Binding of Magnetic Nanoparticles: Implication for detection and magnetic cell separation. Biotechnol and Bioeng. 105(6):1078-1093. 2010. PMID: 20014141;
Wu, Y., Chalmers, J.J., Wyslouzil, B. The use of Electrohydrodynamic Spraying to Disperse Hydrophobic Compounds in Aqueous Media. Aerosol Science. 43(9):902-910. 2009.
Balasubramanian, P., Yang, L., Lang, J.C., Jatana, K.R., Schuller, D., Agrawal, A., Zborowski, M., Chalmers, J.J. Confocal images of circulating tumor cells obtained using a methodology and technology that removes normal cells. Molecular Pharmaceutics 6(5):1402-1408, 2009. PMID: 19445481
Godoy-Silva, R., Chalmers, J.J., Casnocha, SA, Bass, L.A., Ma, N. Physiological Responses of CHO Cells to Repetitive Hydrodynamic Stress. Biotechnol. Bioeng. 103(6):1103-1117. 2009. PMID: 19405151
Shenkman, RM, Chalmers, J.J., Hering, BJ, Kirchhof, N., Papas, K. Quadrupole Magnetic Sorting (QMS) of Porcine Islets of Langerhans. Tissue Engineering Part C Methods. 15(2):147-56. 2009. PMID: 19505179
Shenkman, RM, Godoy-Silva, G., Papas, K., Chalmers, J.J. Effect of Energy Dissipation rate on Islets of Langerhans: Implications for Isolation and Transplantation. Biotechnol and Bioeng. 103:413-423. 2009. PMID: 19191351
Godoy-Silva, R., Mollet, M., Chalmers, J.J., Evaluation of the Effect of Chronic Hydrodynamic Stresses on Cultures of Suspended CHO-6E6 Cells, Biotechnology and Bioengineering. 102(4):1119-1130. 2009. PMID: 18958864
Jing, Y., Nal, Niladri, Williams, P.S., Mayorga, Maritza, Penn, Marc, Chalmers, J. J. Quantitative Intracellular Magnetic Nanoparticle Uptake Measured by Live Cell Magnetophoresis. FASEB Journal.22:4239-4247. 2008. PMID: 18725459
Jin, X., Zhao, Y., Richardson, A., Moore, L., Williams, S., Zborowski, M., Chalmers, J.J. Differences in magnetically induced motion of diamagnetic, paramagnetic, and superparamagnetic microparticles detected by cell tracking velocimetry. The Analyst. 133:1767-1775. 2008. PMID: 19082082
Farson, DF., Choi, HW, Zimmerman, B., Steach, JK, Chalmers, JJ, Olseik, SV, Lee, L.J. Femtosecond laser micromachining of dielectric materials for biomedical applications. J. of Micromechanics and Microengineering. 18(3):035020-035020. 2008.
Mollet, M., Godoy-Silva, R., Berdugo, C., Chalmers, J.J. Computer Simulations of the Energy Dissipation Rate in a Fluorescence Activated Cell Sorter: Implications to Cells. Biotechnol. Bioeng. 100:260-272. 2008. PMID: 18078288
Hu, W., Rathman, J.J., Chalmers, J.J. An Investigation of small molecule surfactants for alleviating bubble associated cell damage. Biotechnol. Bioeng. 101(1):119-127. 2008. PMID: 18646218
Hu, W., Gladue, R., Hansen, J., Wojnar, C., Chalmers, J.J. The Sensitivity of the Dinoflagellate Crypthecodinium cohnii to transient hydrodynamic forces. Biotechnology Progress 23:1355-1362. 2007.
Tong, X., Xiong, Y., Zborowski, M., Farag, S.S. Chalmers, JJ. A Novel High Throughput Immunomagnetic Cell Sorting System for Potential Clinical Scale Depletion of T Cells for Allogeneic Stem Cell Transplantation. Experimental Hematology. 35(10):1613-22. 2007. PMID: 17697744
Tong, X. ,Yang, L., Lang, J. Zborowski, M., Chalmers, J. Application of immunomagnetic cell enrichment in combination with RT-PCR for the detection of rare circulating head and neck tumor cells in human peripheral blood. Cytometry B Clin. Cytom. 72(5):310-323. 2007. PMID: 17205568
Mollet, M., Godoy-Silva, R. Chalmers, JJ. Acute Hydrodynamic Forces Induce Apoptosis: A Complex Question. Biotechnology and Bioengineering 98(4): 772-788. 2007. PMID: 17497730
Mehta, B., Holman, D., Chalmers, J.J., Grzybowsk, D. M. Characterization of arachnoidal cells cultured on three-dimensional non-woven PET matrix. Tissue Engineering. 13(6):1269-79. 2007.
Jing, Y., Moore, LR, Schneider, T., Williams, PS, Chalmers, JJ., Farag, SS, Bolwell, B., Zborowski, M. Negative Selection of Hematopoietic Progenitor Cells by Continuous Magnetophoresis. Experimental Hematology. 35(4):662-672. 2007. PMID: 17379076
Melnik, K., Sun, J., Fleischman, A., Roy, S., Zborowski, M., Chalmers, J.J. Quantification of the Magnetic Susceptibility in several strains of Bacillus spores: implications for detection and separation. Biotechnology and Bioengineering. 98:186-192. 2007.
Schneider T, Moore LR, Jing Y, Haam S, Williams PS, Fleischman AJ, Roy S, Chalmers JJ, Zborowski M., Continuous flow magnetic cell fractionation based on antigen expression level. J Biochem Biophys Methods. 68:1-21. 2006.
Lara, O., Tong, X., Zborowski, M., Farag, S., Chalmers, J.J. Comparison of two Immmunomagnetic Separation Methodologies to Deplete T-cells from human blood samples. Biotechnology and Bioengineering.Vol. 94 (1): 66-80. 2006. PMID: 16518837
Zhang H. Williams PS, Zborowski M, Chalmers JJ. Reduction of binding affinities/avidities of Antibody-Antigen and Strepaddvidin-biotin: Quantification and Scale-up implications. Biotechnol. Bioeng. 95:812-829. 2006.
Leigh, D.R., Steinert, S., Moore, L., Chalmers, J.J., Zborowski, M., Cell Tracking Velocimetry as a Tool for Defining Saturation Binding of Magnetically Conjugated Antibodies. Cytometry A. Cytometry A. 66(2):103-8. 2005
Carpino, F., Moore, L.R., Zborowski, M., Chalmers, J.J., Williams, P.S., Analysis of magnetic nanoparticles using quadrupole magnetic field-flow fractionation. Journal of Magnetism and Magnetic Materials 293:546-552. 2005.
Carpino F, Moore LR, Chalmers JJ, Zborowski M, Williams PS. Quadrupole magnetic field-flow fractionation for the analysis of magnetic nanoparticles. ICFPM Proceedings in Journal of Physics: Conference Series 2005.
Zhang, H, Zborowski, M., Williams, P S., Chalmers, J. Establishment and implications of a characterization method for magnetic nanoparticles using Cell Tracking Velocimetry and magnetic susceptibility modified solutions. Analyst, 130:514 – 527. 2005. PMID: 15776162
Moore, L.R., Milliron, L., Williams, P.S., Chalmers, J.J., Margel, S., Zborowski, M., Control of magnetophoretic mobility by susceptibility-modified solutions as evaluated by cell tracking velocimetry and continuous magnetic sorting. Analytical Chem. 76:3899-3907. 2004
Ma, N., Chalmers, J.J., Aunins, J.G., Zhou, W., Xie, L Quantitative Studies of Cell Damage in Sparged Bioreactors Using A Bubble Collector to Segregate Bubble Rupture Events, Biotechnology Progress 20(4):1183 – 1191. 2004. PMID: 15296446
Fife, J.P., Derksen, R.C., Ozkan, H.E., Grewal, P.S., Chalmers, J.J. Evaluation of a Contraction Flow Field on Hydrodynamic Damage to Entompathogenic Nematodes- a Biological Pest Control Agent. Biotechnology and Bioengineering 86(1):11:96-107. 2004.
Williams, S., Decker, K., Nakamura, M., Chalmers, J.J., Moore, L., Zborowski, M. Splitter Imperfections in Annular Split-Flow Thin Separation Channels: Experimental Study of Nonspecific Crossover. Analytical Chemistry, 75(23):6687-6695. 2003.
Williams, S., Moore, L.R., Chalmers, J.J., Zborowski, M. Splitter Imperfections in Annular Split-Flow Thin Channel Separations of Cells: Effect of Non-Specific Crossover. Analytical Chemistry 75(6):1365-1373. 2003.
McCloskey, K. Moore, L., Hoyos, M., Rodrigues, A., Chalmers, J.J., Zborowski, M. Magnetic cell separation is a function of antibody binding capacity (ABC). Biotechnology Progress 19(3):899-907. 2003. PMID: 12790655.
Chosy, J., Melnik, K., Comella, K., Zborowski, M., Chalmers, J.J. Characterization of Antibody Binding to Three Cancer-Related Antigens Using Flow Cytometry and Cell Tracking Velocimetry. Biotechnology and Bioengineering 82:340-351. 2003.
Zborowski, M., Moore, L.R., Williams, S. Chalmers, J.J. Separations based on magnetophoretic mobility, Separation Sciences and Technology, 37: 3611-3633. 2002.
Hoyos, M., McCloskey, K., Moore, L., Nakamura, M., Bolwell, B., Chalmers, J., Zborowski, M. Pulse-injection studies of blood progenitor cells in a quadrupole magnetic flow sorter. J. Separation Science and Technology 37(4) 1-23. 2002.
Comella, K., Melnik, K., Chosy, J., Zborowski, M., Cooper, M.A., Fehniger, T.A. Caligiuri, M.A., and Chalmers, J.J. The effect of antibody concentration on the separation of human natural killer cells in a commercial immuno-magnetic separation system. Cytometry 45:285-293. 2001.
Melnik, K. Nakamura, M., Comella, K., Zborowski, M., Chalmers, J.J. Evaluation of eluants from batch separations of CD34(+) cells from human cord blood using a commercial, immunomagnetic cell separation system. Biotechnol Progress 17(5):907-916. 2001.
McCloskey, K., Comella, K., Margel, S., Chalmers, J., Zborowski, M. Mobility measurements of immunomagnetically labeled cells allow quantitation of secondary antibody binding amplification. Biotechnol. Bioeng. 75:642-655. 2001. PMID: 11378865.
Nakamura, M., Decker, K., Chosy, J., Comella, K., Melnik, K. Moore, L., Zborowski, M., Chalmers, J.J. Separation of Breast Cancer Cells by Quadrupole Magnetic Flow Sorter, Biotechnology.Progress 17:1145-1155. 2001. PMID: 11735453
McCloskey, K., Chalmers, J.J., Zborowski, M., Measurement of CD2 Expression Levels of IFN-" Treated Fibrosarcomas Using Cell Tracking Velocimetry, Cytometry. Jun 1;44(2):137-47. 2001. PMID: 11378865.
Moore, L.R., Rodrigues, A.R., Williams, P.S., McCloskey, K., Bolwell, B.J., Nakamura, M., Chalmers, J.J., Zborowski, M. Progenitor cell isolation with a high-capacity quadrupole magnetic flow sorter. J. Magn. Magn. Mater. 225(1-2):277-284. 2001
Nakamura, N., Lasky, L., Zborowski, M., Chalmers, J.J. Theoretical and Experimental Analysis of the Accuracy of Cell Tracking Velocimetry, Experiments in Fluids 30:371-380. 2001.
Hoyos, M., Moore, L.R., McCloskey, K.E., Margel S., Zuberi, M., Chalmers, J.J., Zborowski, M. Study of magnetic particles pulsed-injected into an annular SPLITT-like channel inside a quadrupole magnetic field, J. Chromatogr., A , 903(1-2):99-116. 2000. PMID: 1153960.
Moore, L.R. Sun, L.P., Zborowski, M., Nakamura, M., Chalmers, J.J., McCloskey, K. Gura, S., Burdygin, I., Margel, S. The use of Magnetite-Doped Polymeric Microspheres in Calibrating Cell Tracking Velocimetry, J. Biochemical and Biophysical Methods 44:115-130, 2000.
McCloskey, K. E., Zborowski, M., Chalmers, J.J. Magnetophoretic Mobilities Correlate to Antibody Binding Capacity, Cytometry 40:307-315. (2000). Erata, Cytometry 41: (2)150-150, OCT 1 2000. PMID: 10918281.
Bauer, T., Hancock, L., Rathman, J., Chalmers, J.J., Cell-Microcarrier adhesion to Gas-Liquid Interfaces and Foam, Biotechnology Progress 16:125-132, 2000.
Williams, P.S., Zborowski, M., Chalmers, J.J. Flow Rate for the Quadrupole Magnetic Cell Sorter. Analytical Chemistry 71:3799-3807, 1999.
Chalmers, J.J., Haam, S., Zhou, Y., McCloskey, K., Moore, L., Zborowski, M., Williams, P.S. Quantification of Cellular Properties from External Fields and Resulting Induced Velocity: Cellular Hydrodynamic Diameter, Biotechnology and Bioengineering 64:509-518, 1999.
Chalmers, J.J., Haam, S., Zhou, Y., McCloskey, K., Moore, L., Zborowski, M., Williams, P.S. Quantification of Cellular Properties from External Fields and Resulting Induced Velocity: Magnetic Susceptibility, Biotechnology and Bioengineering 64:519-526, 1999.
Zborowski, M., Sun, L., Moore, L.R., Chalmers, J.J. Rapid cell isolation by magnetic flow sorting for applications in tissue engineering, ASIO Journal 45:127-130, 1999.
Moore, L.R., Zborowski, M., Sun, L. Chalmers, J.J. Lymphocyte fractionation using immunomagnetic colloid and dipole magnet flow cell sorter, J. Biochemical and Biophysical Methods 37:11-33, 1998.
Chalmers, J.J., Gas Bubbles and their Influence on Microorganisms, Applied Mechanics Reviews, Vol. 51: 113-121, 1998.
Wheeler, G., Chalmers, J.J., Tomasko, D., A Linked Supercritical Extraction-Biodegradation System to Extract and Degrade Phenol, Chemical Engineering Science. 53:189-201, 1998.
Chalmers, J.J, Mandal, S., Fang, B., Sun, L., Zborowski, M., Theoretical Analysis of Cell Separation Based on Cell Surface Marker Density, Biotechnology and Bioengineering 59:10-20 (1998).
Sun, L., Zborowski, M., Moore, L., Chalmers, J.J., Continuous, Flow-Through Immunomagnetic Cell Separation in a Quadrupole Field. Cytometry. 33:469-475. 1998.
Vir, R., Chalmers, J.J., A Confocal Microscopic Observation of Non-Submerged Biofilms, Biotechnology Progress. 13:727-732, 1997.
Zborowski, M., Williams, P.S., Sun. L., Moore, L.R., Chalmers, J.J., Cylindrical Split and Quadrupole Magnetic Field in Applications to Continuous-Flow Magnetic Cell Sorting, J. Liquid Chromatography and Related Techniques.20:2887-2905, 1997.
Chalmers, Jeffrey J.J., Shear sensitivity of insect cells, Cytotechnology.20:163-171, 1996.
Tseng, D.Y., Vir, R., Traina, S.J., Chalmers, J.J., A Fourier-Transform Infrared Spectroscopic Analysis of Organic Matter Degradation in a Bench-Scale Solid Substrate Fermentation (Composting) System", Biotechnology and Bioengineering. 52:661-671. (1996).
Tseng, D.Y., Tuovinen, O.H., and Chalmers, J.J. ATP measurements in Compost, Compost Science and Utilization, 4:6-17, 1996.
Zborowski, M., Fuh, C.B., Green, R., Baldwin, N.J., Reddy, S., Douglas, T., Mann, S., Chalmers, J.J., Immunomagnetic Isolation of Magnetoferritin-Labeled Cells in a Modified Ferrograph, Cytometry 24:251-259, 1996.
Venkat, R., Stock, R., Chalmers, J.J. Study of Hydrodynamics in Microcarrier Culture Spinner Vessels: A Particle Tracking Velocimetry Approach, Biotechnology and Bioengineering 49:456-466, 1996.
Reddy, S., Moore, L., Zborowski, M., Chalmers, J.J., Determination of the Magnetic Susceptibility of Labeled Particles by Video Imaging, Chemical Engineering Science 51:947-956, 1996.
Venkat, R.V., Chalmers, J.J., Characterization of agitation environments in 250 ml spinner vessel, 3 L, and 20 L reactor vessel used for animal cell microcarrier culture. Cytotechnology. 22:95-102, 1996.
Tseng, D.Y., Tuovinen, O.H., Hoitink, H.A. J., Chalmers, J.J. Characterization of a Bench-Scale System for Studying the Biodegradation of Organic Solid Wastes, Biotechnology Progress, 11:443-451, 1995.
Chattopadhyay, D., Rathman, J., Chalmers, J.J., A Thermodynamic Explanation for Cell Adhesion to Gas-Liquid Interfaces, Biotechnology and Bioengineering, 48:649-658, 1995.
Chattopadhyay, D., Rathman, J., Chalmers, J.J., The Protective Effect of Specific Medium Additives with Respect to Bubble Rupture, Biotechnology and Bioengineering. 45:473-480, 1995.
Garcia-Briones, M.A., Brodkey, R.S., Chalmers, J.J., Computer Simulations of the Rupture of a Gas Bubble at a Gas-Liquid Interface and its Implications in Animal Cell Damage, Chemical Engineering Science, 49:2301-2320, 1994.
Chalmers, J.J., Cells and Bubbles in Sparged Bioreactors, Cytotechnology, 15:311-320, 1994.
Garcia-Briones, M.A, Chalmers, J.J., Flow Parameters Associated with Hydrodynamic Cell Injury, Biotechnology and Bioengineering, 44:1089-1098, 1994.
Trinh, K., Garcia-Briones, M., Hink, F., Chalmers, J.J. Quantification of Suspended Cell Damage as a Result of Bubble Rupture, Biotechnology and Bioengineering. 43:37-45, 1994.
Garcia-Briones, M.A., Chalmers, J.J., Cell-Bubble Interactions: Mechanisms of Suspended Cell Damage, Annals of the New York Academy of Sciences, 665:219-229, 1992.
Kleman, G., Chalmers, J.J., Strohl, W., Glucose-stat: A Predictive and Feedback Controlled Continuous Culture, Applied and Environmental Microbiology, 57:918-923, 1991.
Kleman, G., Chalmers, J.J., Strohl, W. Predictive and Feedback Control Provides Constant Glucose Concentration in Fed-batch Fermentations, Applied and Environmental Microbiology, 57:910-917, 1991.
Goldblum, S.D., Bae, Y.K., Hink, W., Chalmers, J.J., The Protective Effect of Methylcellulose on Suspended Insect Cells Subjected to Laminar Shear Stress, Biotechnology Progress, 6:383-390, 1990.
Chalmers, J.J., Kim, E., Telford, J.N., Shuler, M.L., Wilson, D.B., Effects of Temperature on the Stability of Escherichia coli Overproducing β-lactamase or Human Epidermal Growth Factor, Applied and Environmental Microbiology, 56:104-111, 1990.
Chalmers, J.J., Shuler, M.L., Wilson, D.B., A Simple Membrane Reactor to Obtain High Cell Density and Plasmid Encoded Protein Production from Escherichia coli, Biotechnology Techniques, 4:73-78, 1990.
Georgiou, G., Chalmers, J.J., Shuler, M.L., Wilson, D.B., Continuous Immobilized Recombinant Protein Production from E. coli Capable of Selective Protein Excretion: A Feasibility Study, Biotechnology Progress, 1:75-79, 1985.
Arnold, F.H., Chalmers, J.J., Saunders, M.S., Croughan, M.S., Blanch, H.W., Wilke, C.R., A Rational Approach to the Scale-up of Affinity Chromatography", A.C.S. Symposium Series 271, p. 113 (1985).

1. Chalmers JJ, Melnik K, Lara-Velasco, OR, Tong X, Zborowski M, Moore LR, Farag S. Biological Cell Separator and Disposable Kit. US Patent 9,176,111; 2015

2. Sooryakumar, Ratnasingham; Henighan, Thomas Charles; Vieira, Gregory B.; Chalmers, Jeffrey J. Magnetic platforms for biomolecule trappings, manipulations, and sorting, Patent Number :US 08906691. December 9, 2014.

3. Sooryakumar, Ratnasingham; Sooryakumar, Dhriti; Vieira, Gregory; Chalmers, Jeffrey J. Mobile magnetic traps and platforms for micro/nano particle manipulation. U. S. Patent 6,623,984, March 19, 2013

4. Chalmers, J.J, Wu, W, Rathman, Compositions for reducing cell adhesion to bubbles. U.S. Patent 08440800 May 14, 2013.

5. Fleischman, A., Roy, S., Chalmers, J., Zborowski, M. MEMS-BASED Integrated Magnetic Particle Identification System. U. S. Patent 6,623,984, Sept. 2003.

6. Zborowski, M., Chalmers, J.J., Moore, L. System and device for determining particle characteristics. U.S. Patent 6,557,430, May 2003

7. Zborowski, M., Chalmers, J.J., Moore, L. System and Device for determining particle characteristics. U.S. Patent 6,412,359, October, 2002.

8. Zborowski M, Chalmers PS, Moore LR. Continuous particle and molecule separation with an annular flow channel, U.S. Patent 6,467,630, October 2002.

9. Zborowski, M., Chalmers, J.J. Moore, L. System and Device for Determining Particle Characteristics, U.S. Patent 6,082,205; 2000.

10. Zborowski, M., Chalmers, J.J. Moore, L. Fractional cell sorter, U.S. Patent 6,120,735, 2000.

11. Zborowski, M., Chalmers, J.J. Moore, L. Method for determining particle characteristics , U.S. Patent 6,142,025, 2000.

12. Zborowski, M., Chalmers, J.J. Moore, L., Method for magnetically separating cells into fractionated flow streams, U.S. Patent 5,968,820, 1999.

13. Zborowski, M., Chalmers, J.J. Moore, L. Method for Determining Particle Characteristics, U.S. Patent 5,974,901, 1999.

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